Sears M L, Yamada E, Cummins D, Mori N, Mead A, Murakami M
Yale University School of Medicine, Department of Ophthalmology and Visual Science, New Haven, CT.
Trans Am Ophthalmol Soc. 1991;89:131-52; discussion 152-4.
An intact ciliary epithelial bilayer has been isolated from the rabbit eye by perfusion, microsurgical dissection, and recovery techniques. Vital subcellular organelles and intercellular junctions of this epithelial bilayer preparation are very well preserved. The total electrical resistance of the epithelial bilayer is 350 ohms, and the transepithelial potential is 650 microV, nonpigmented epithelium side negative. The electrical resistance is reduced by 0.2 mM EGTA and the transepithelial potential reduced by 0.1 mM ouabain. Bicarbonate depletion at a constant pH of 7.4 rapidly and significantly reduces the transepithelial potential. Carbonic anhydrase inhibitors decrease transmembrane potential by as much as 30%. These morphologic and physiologic experiments authenticate the validity of this bilayered epithelial preparation for future use in detailed studies of the mechanism of aqueous humor formation.
通过灌注、显微外科解剖和回收技术，已从兔眼中分离出完整的睫状体上皮双层结构。这种上皮双层结构制剂的重要亚细胞器和细胞间连接保存得非常完好。上皮双层的总电阻为350欧姆，跨上皮电位为650微伏，无色素上皮侧为负。0.2 mM的乙二醇双四乙酸（EGTA）可降低电阻，0.1 mM的哇巴因可降低跨上皮电位。在pH值恒定为7.4的情况下，碳酸氢盐耗尽会迅速且显著地降低跨上皮电位。碳酸酐酶抑制剂可使跨膜电位降低多达30%。这些形态学和生理学实验证实了这种双层上皮制剂在未来用于详细研究房水形成机制方面的有效性。
