Zhang Xiao-Hui, Hu Yu, Shen Guan-Xin, Wei Wen-Ning, Song Shan-Jun
Institute of Hematology and People Hospital, Peking University, Beijing 100044, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Dec;15(6):1191-5.
The aim of this study was to investigate the gene expression profiles of acute promyelocytic leukemia (APL) cell line NB4 treated with arsenic trioxide (As2O3) by using cDNA microarray. cDNA probes were prepared through reverse transcription from mRNA of NB4 cells treated with or without arsenic trioxide. The probes were labeled with Cy3 and Cy5 fluorescence dyes individually, hybridized with cDNA microarray representing 201 different human genes, and their fluorescent intensities were scanned. The genes were screened through the analysis of the difference in the gene expression profile. The results showed that after the treatment of arsenic trioxide (2 micromol/L), 6 genes were up-regulated, and 12 genes related to apoptosis and signal transduction were down-regulated. The p21, survivin, cdc2 and Wee1Hu genes may be related to the differentiation and/or apoptosis of NB4 cells induced by As2O3. It is concluded that p21, survivin, cdc2 and Wee1Hu may play an important role in the mechanism underling arsenic trioxide-mediated NB4 cell apoptosis.
本研究旨在利用cDNA微阵列技术研究经三氧化二砷（As2O3）处理的急性早幼粒细胞白血病（APL）细胞系NB4的基因表达谱。通过对经或未经三氧化二砷处理的NB4细胞的mRNA进行逆转录制备cDNA探针。将探针分别用Cy3和Cy5荧光染料标记，与代表201种不同人类基因的cDNA微阵列杂交，并扫描其荧光强度。通过分析基因表达谱的差异来筛选基因。结果显示，经三氧化二砷（2微摩尔/升）处理后，6个基因上调，12个与凋亡和信号转导相关的基因下调。p21、survivin、cdc2和Wee1Hu基因可能与三氧化二砷诱导的NB4细胞分化和/或凋亡有关。结论是，p21、survivin、cdc2和Wee1Hu可能在三氧化二砷介导的NB4细胞凋亡机制中发挥重要作用。
