Aslan Ahmet, Temiz Muhyittin, Hakverdi Sibel, Polat Gurbuz, Tumer Cemil, Temiz Abdulkerim, Canbolant Elif
Mustafa Kemal University, Faculty of Medicine, Department of General Surgery, Antakya-Hatay, Turkey.
Int J Surg. 2008 Feb;6(1):40-4. doi: 10.1016/j.ijsu.2007.09.003. Epub 2008 Feb 20.
We aimed to investigate the effect of mesalamine on healing of experimental colon anastomosis model.
MATERIAL/METHODS: Forty adult male Wistar albino rats were performed segmentary colonic resection and end-to-end anastomosis. Animals were randomly divided into four groups: group I, anastomosis group, received no treatment (GI, n=8); group II, anastomosis+oral mesalamine group (100mg/kg/day); group III, anastomosis+rectal mesalamine (2mL) group, (GIII, n=8); group IV, anastomosis+oral mesalamine+rectal mesalamine (GIV, n=8) group. A sham group (n=8) was constituted and was performed laparotomy. Bursting pressure, hydroxyproline levels and histopathological characteristics of the anastomosis were analyzed.
Although it was not statistically significant, there was an increase in the burst pressure of the mesalamine group. When hydroxyproline measurements were compared there were statistically significant difference between the non-treated colon and all groups. There were significant differences between GI and GIII-GIV, GII and GIV. The differences between group I and II and group II and III were not statistically significant. When we compared the median amount of the histopathological changes, we found significant difference between the anastomosis and the mesalamine groups (P<0.05). But when mesalamine groups were compared with each other we did not observe a significant difference.
Mesalamine had positive effects which were not statistically significant on bursting pressure and statistically different significant effects on hydroxyproline (HP) levels based on the way of administration and statistically significant positive effects on histopathologic anastomotic healing in experimental anastomosis model.
我们旨在研究美沙拉嗪对实验性结肠吻合模型愈合的影响。
材料/方法：对40只成年雄性Wistar白化大鼠进行节段性结肠切除和端端吻合。动物被随机分为四组：第一组，吻合组，不接受治疗（GI，n = 8）；第二组，吻合+口服美沙拉嗪组（100mg/kg/天）；第三组，吻合+直肠美沙拉嗪（2mL）组（GIII，n = 8）；第四组，吻合+口服美沙拉嗪+直肠美沙拉嗪（GIV，n = 8）组。设立假手术组（n = 8）并进行剖腹手术。分析吻合口的破裂压力、羟脯氨酸水平和组织病理学特征。
虽然无统计学意义，但美沙拉嗪组的破裂压力有所增加。当比较羟脯氨酸测量值时，未治疗的结肠与所有组之间存在统计学显著差异。GI与GIII - GIV、GII与GIV之间存在显著差异。第一组与第二组以及第二组与第三组之间的差异无统计学意义。当比较组织病理学变化的中位数时，我们发现吻合组和美沙拉嗪组之间存在显著差异（P<0.05）。但当美沙拉嗪组相互比较时，未观察到显著差异。
在实验性吻合模型中，美沙拉嗪对破裂压力有积极但无统计学意义的影响，对羟脯氨酸（HP）水平根据给药方式有统计学显著差异的影响，对组织病理学吻合口愈合有统计学显著的积极影响。
