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Mol Cell Proteomics. 2007 Jul;6(7):1123-34. doi: 10.1074/mcp.M700001-MCP200. Epub 2007 Apr 19.
3
A novel quantitative proteomics reagent based on soluble nanopolymers.
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A comparative analysis of the clotting and fibrinolytic activities of the snake venom (Bothrops atrox) from different geographical areas in Venezuela.委内瑞拉不同地理区域的蛇毒（矛头蝮）凝血和纤溶活性的比较分析。
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使用基于可溶性聚合物的同位素标记对蛇毒进行定量分析。

Quantitative analysis of snake venoms using soluble polymer-based isotope labeling.

作者信息

Galan Jacob A, Guo Minjie, Sanchez Elda E, Cantu Esteban, Rodriguez-Acosta Alexis, Perez John C, Tao W Andy

机构信息

Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.

出版信息

Mol Cell Proteomics. 2008 Apr;7(4):785-99. doi: 10.1074/mcp.M700321-MCP200. Epub 2007 Dec 18.

DOI:10.1074/mcp.M700321-MCP200
PMID:18089550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2401333/
Abstract

We present the design and synthesis of a new quantitative strategy termed soluble polymer-based isotope labeling (SoPIL) and its application as a novel and inclusive method for the identification and relative quantification of individual proteins in complex snake venoms. The SoPIL reagent selectively captures and isolates cysteine-containing peptides, and the subsequent tagged peptides are released and analyzed using nanoflow liquid chromatography-tandem mass spectrometry. The SoPIL strategy was used to quantify venom proteins from two pairs of venomous snakes: Crotalus scutulatus scutulatus type A, C. scutulatus scutulatus type B, Crotalus oreganus helleri, and Bothrops colombiensis. The hemorrhagic, hemolytic, clotting ability, and fibrinogenolytic activities of crude venoms were measured and correlated with difference in protein abundance determined by the SoPIL analysis. The SoPIL approach could provide an efficient and widely applicable tool for quantitative proteomics.

摘要

我们展示了一种名为基于可溶性聚合物的同位素标记（SoPIL）的新定量策略的设计与合成，及其作为一种新颖且全面的方法用于复杂蛇毒中单个蛋白质的鉴定和相对定量的应用。SoPIL试剂选择性地捕获和分离含半胱氨酸的肽段，随后释放标记的肽段并使用纳流液相色谱 - 串联质谱进行分析。SoPIL策略用于定量两对毒蛇的毒液蛋白：A 型盾鳞响尾蛇（Crotalus scutulatus scutulatus）、B 型盾鳞响尾蛇（C. scutulatus scutulatus）、俄勒冈州南部响尾蛇（Crotalus oreganus helleri）和哥伦比亚矛头蝮（Bothrops colombiensis）。测定了粗毒液的出血、溶血、凝血能力和纤维蛋白溶解活性，并将其与通过SoPIL分析确定的蛋白质丰度差异相关联。SoPIL方法可为定量蛋白质组学提供一种高效且广泛适用的工具。