Tao W Andy
Purdue University, Department of Biochemistry, Purdue Cancer Center, West Lafayette, IN 47907, USA.
Expert Rev Proteomics. 2007 Oct;4(5):603-7. doi: 10.1586/14789450.4.5.603.
Much attention has been given to protein biomarker discovery in the field of proteomics in the past few years. Proteomic strategies for biomarker discovery normally include the identification of proteins that alter during the progression of a particular disease state in high throughput. To perform these studies requires the ability to measure changes of low-abundance proteins in highly complex mixtures from different biological states. Soluble polymer-based isotope labeling (SoPIL) is a new proteomics strategy that targets specific classes of proteins for isotopic labeling, efficient isolation and accurate quantitation by mass spectrometry. The method exploits the features of homogenous solution-phase reaction, simple solid-phase extraction and characteristic cell-permeable nanoparticles. Recent applications demonstrate that the SoPIL reagents are ideal for quantitative proteomics and phosphoproteomics, and could have the potential to discover disease markers in the most physiologically relevant settings.
在过去几年里，蛋白质组学领域对蛋白质生物标志物的发现给予了极大关注。用于生物标志物发现的蛋白质组学策略通常包括高通量鉴定在特定疾病状态进展过程中发生变化的蛋白质。要开展这些研究，需要具备测量来自不同生物状态的高度复杂混合物中低丰度蛋白质变化的能力。基于可溶性聚合物的同位素标记（SoPIL）是一种新的蛋白质组学策略，它针对特定类别的蛋白质进行同位素标记、高效分离并通过质谱进行准确定量。该方法利用了均相溶液相反应、简单的固相萃取以及具有细胞渗透性的特征纳米颗粒的特性。最近的应用表明，SoPIL试剂对于定量蛋白质组学和磷酸化蛋白质组学是理想的，并且有可能在最生理相关的环境中发现疾病标志物。
