Mela Christopher M, Steel Alan, Lindsay James, Gazzard Brian G, Gotch Frances M, Goodier Martin R
Department of Immunology, Imperial College London, UK.
AIDS. 2007 Oct 18;21(16):2177-82. doi: 10.1097/QAD.0b013e3282f08b72.
HIV-1 infection is known to have a detrimental impact on peripheral blood natural killer cell phenotype and function. Chronic HIV-1 also causes a substantial depletion of CD4+ T cells in the gastrointestinal tract and the blood.
To investigate the impact of chronic HIV-1 infection with on natural killer cell populations in the gastrointestinal tract and the effect of suppression of plasma viraemia with antiretroviral therapy.
Lymphocyte populations were extracted from the lamina propria of biopsies taken from the sigmoid colon of HIV-1-infected and uninfected individuals. The proportions of natural killer cell subsets were compared in viraemic (n = 15) and aviraemic HIV-1-positive, HAART-treated individuals (n = 27) and HIV-1 negative control individuals (n = 26) using flow cytometry on gated subsets.
Natural killer cells are depleted in colonic biopsies from HIV-1-infected individuals with detectable plasma virus in comparison with HIV-1-negative individuals. A significant increase in the proportion of both natural killer and CD4+ T cells in the colonic lamina propria is observed in aviraemic individuals compared to viraemic individuals.
Chronic HIV-1 infection results in depletion of both natural killer cells and CD4+ T cells in colonic tissue and antiretroviral therapy results in a recovery of these subsets in individuals with undetectable plasma viral load.
已知HIV-1感染对外周血自然杀伤细胞的表型和功能有不利影响。慢性HIV-1感染还会导致胃肠道和血液中CD4+T细胞大量耗竭。
研究慢性HIV-1感染对胃肠道自然杀伤细胞群体的影响以及抗逆转录病毒疗法抑制血浆病毒血症的效果。
从HIV-1感染个体和未感染个体乙状结肠活检标本的固有层中提取淋巴细胞群体。使用流式细胞术对门控亚群进行分析，比较病毒血症（n = 15）和无病毒血症的HIV-1阳性、接受高效抗逆转录病毒治疗的个体（n = 27）以及HIV-1阴性对照个体（n = 26）中自然杀伤细胞亚群的比例。
与HIV-1阴性个体相比，血浆病毒可检测的HIV-1感染个体的结肠活检标本中自然杀伤细胞减少。与病毒血症个体相比，无病毒血症个体的结肠固有层中自然杀伤细胞和CD4+T细胞的比例均显著增加。
慢性HIV-1感染导致结肠组织中自然杀伤细胞和CD4+T细胞耗竭，抗逆转录病毒疗法可使血浆病毒载量不可检测的个体中这些亚群得以恢复。
