Wu Zi-xiang, Wei Lei, Hu Yun-yu, Wang Hai-qiang, Wan Shi-yong, Wang Jun, Han Yisheng
Institude of Orthopaedics, Xijing Hospital, the Fourth Military Medical University, Xi'an Shaanxi Province, PR China.
Spine (Phila Pa 1976). 2007 Oct 15;32(22):2437-42. doi: 10.1097/BRS.0b013e3181573976.
Fibrin sealant (FS) combined with or without growth factor was used to improve the micro-architectural and biomechanical properties of vertebral body in osteoporotic ovine spine.
To analyze the treatment effects of bovine bone morphogenetic protein (bBMP) combined with FS on osteopenic ovine vertebral architecture, bone mineral density, and biomechanics in vivo.
Vertebroplasty and kyphoplasty were used to treat spinal osteoporosis. They can increase strength of vertebrae physically. However, each has specific disadvantages. bBMP could rapidly increasing bone formation and suppressing bone resorption, but little is known about its effect on ovariectomized-induced osteoporosis.
Six sheep underwent ovariectomy and were placed on a low-calcium diet. Twelve months later, according to Ladin square design, L4-L6 vertebrae in all sheep were randomly assigned to 3 treatment groups: A (30 mg bBMP/1.5 mL FS), B (30 mg bBMP) and C (1.5 mL FS). All materials were injected into the assigned vertebra transpedicularly. Animals were killed 3 months after injection, and bone mineral density (BMD), biomechanics, and histomorphometry were assessed. Analysis of variance was used to determine effects of bBMP/FS (alpha = 0.05).
The BMD in Group 1 was 17.1% and 14.7% higher than that in Group 2 and Group 3, respectively. The micro-CT reconstruction analysis showed that the density and connectivity of trabecular bone in bBMP/FS treated vertebrae were higher than those in control groups. The mechanical properties (yield stress, ultimate stress, energy absorption, bone modulus) of the vertebrae were also significantly higher. In this study, local bBMP/FS treatment showed a positive trend in improving BMD, histomorphometric parameters, and mechanical strength of osteoporotic vertebra. Slow release of bBMP using FS appeared to be an effective method of protein delivery.
The use of bBMP/FS in the treatment of vertebral osteoporosis in an attempt to enhance bone strength merits further study.
使用含或不含生长因子的纤维蛋白密封剂（FS）来改善骨质疏松绵羊脊柱椎体的微观结构和生物力学性能。
分析牛骨形态发生蛋白（bBMP）联合FS对骨质疏松绵羊椎体结构、骨密度及生物力学的体内治疗效果。
椎体成形术和后凸成形术用于治疗脊柱骨质疏松症。它们能物理性增加椎体强度。然而，每种方法都有特定缺点。bBMP可快速增加骨形成并抑制骨吸收，但对其对去卵巢诱导的骨质疏松症的影响了解甚少。
6只绵羊接受去卵巢手术并给予低钙饮食。12个月后，根据拉丁方设计，将所有绵羊的L4-L6椎体随机分为3个治疗组：A组（30mg bBMP/1.5mL FS）、B组（30mg bBMP）和C组（1.5mL FS）。所有材料经椎弓根注入指定椎体。注射后3个月处死动物，评估骨密度（BMD）、生物力学和组织形态计量学指标。采用方差分析确定bBMP/FS的作用（α=0.05）。
第1组的骨密度分别比第2组和第3组高17.1%和14.7%。显微CT重建分析显示，bBMP/FS治疗的椎体中松质骨的密度和连通性高于对照组。椎体的力学性能（屈服应力、极限应力、能量吸收、骨模量）也显著更高。在本研究中，局部bBMP/FS治疗在改善骨质疏松椎体的骨密度、组织形态计量学参数和力学强度方面呈阳性趋势。使用FS实现bBMP的缓释似乎是一种有效的蛋白质递送方法。
使用bBMP/FS治疗椎体骨质疏松症以增强骨强度值得进一步研究。
Zotero 插件