Wu Zi Xiang, Liu Da, Wan Shi Yong, Cui Geng, Zhang Yang, Lei Wei
Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, No. 17 Changlexi Road, Xi'an, 710032, Shaanxi, People's Republic of China.
J Orthop Sci. 2011 Jan;16(1):99-104. doi: 10.1007/s00776-010-0019-x. Epub 2011 Feb 4.
The purpose of this study was to analyze the local treatment effects of rhBMP-2 combined with fibrin sealant (FS) on bone mineral density, microarchitectural and mechanical properties in osteoporotic ovine spine.
Postmenopausal osteoporosis was induced in eight sheep through ovariectomy (OVX) and a low-calcium diet for a period of 12 months. According to the Latin square design, L3-L6 vertebrae were randomly assigned to four treatment groups: A (rhBMP-2/FS), B (rhBMP-2), C (FS) and D (blank control). All materials were injected into the assigned vertebra transpedicularly. All animals were euthanized 3 months after treatment. Bone mineral density (BMD), microarchitectural and mechanical properties were assessed. ANOVA analysis of variance was used to determine effects of rhBMP-2/FS (α = 0.05).
The BMD in group A (rhBMP-2/FS) was 18.8, 30.4 and 27.9% higher than that in group B, C and D, respectively. Analysis of bone structure by micro-CT revealed higher trabecular bone volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N) in the rhBMP-2/FS group (P < 0.01). In addition, vertebrae treated with rhBMP-2/FS exhibited higher yield stress, ultimate stress, energy absorption and bone modulus compared to the control groups.
Local administration of rhBMP-2/FS showed a positive trend in improving BMD, microarchitectural parameters and mechanical strength of osteoporotic vertebra. Slow release of rhBMP-2 using FS appeared to be an effective method of protein delivery. The local treatment of osteoporosis in the spine can increase bone strength and reduce fracture risk quickly.
本研究旨在分析重组人骨形态发生蛋白-2（rhBMP-2）联合纤维蛋白密封剂（FS）对骨质疏松绵羊脊柱骨密度、微观结构及力学性能的局部治疗效果。
通过卵巢切除术（OVX）及低钙饮食12个月诱导8只绵羊发生绝经后骨质疏松。根据拉丁方设计，将L3-L6椎体随机分为4个治疗组：A组（rhBMP-2/FS）、B组（rhBMP-2）、C组（FS）和D组（空白对照）。所有材料经椎弓根注入指定椎体。治疗3个月后对所有动物实施安乐死。评估骨密度（BMD）、微观结构及力学性能。采用方差分析（ANOVA）确定rhBMP-2/FS的作用效果（α = 0.05）。
A组（rhBMP-2/FS）的骨密度分别比B组、C组和D组高18.8%、30.4%和27.9%。通过显微CT分析骨结构发现，rhBMP-2/FS组的骨小梁体积（BV/TV）、骨小梁厚度（Tb.Th）和骨小梁数量（Tb.N）更高（P < 0.01）。此外，与对照组相比，接受rhBMP-2/FS治疗的椎体表现出更高的屈服应力、极限应力、能量吸收和骨模量。
局部应用rhBMP-2/FS在改善骨质疏松椎体的骨密度、微观结构参数和力学强度方面呈现出积极趋势。使用FS实现rhBMP-2的缓释似乎是一种有效的蛋白质递送方法。脊柱骨质疏松的局部治疗可快速增强骨强度并降低骨折风险。
