Vincent Kathleen L, Bell Brent A, Rosenthal Susan L, Stanberry Lawrence R, Bourne Nigel, Sweeney Yvonne T Cosgrove, Patton Dorothy L, Motamedi Massoud
University of Texas Medical Branch, Galveston, Texas 77555-0319, USA.
Sex Transm Dis. 2008 Mar;35(3):269-75. doi: 10.1097/OLQ.0b013e31815abad8.
Safety is a primary concern in the development of topical microbicides. Optical coherence tomography (OCT), a high-resolution, in-depth cross-sectional imaging modality, was utilized in conjunction with colposcopy to assess induced cervicovaginal epithelial changes that may predict product safety.
OCT and colposcopic images of macaque vaginal and cervical tissues were obtained in excised tissue and in vivo under various conditions, including mechanical injury and nonoxynol-9 treatment.
A scoring system was developed to categorize and quantify the OCT images based on morphologic features that indicate the presence or absence of an intact epithelial layer and inflammation. Using 3 categories (normal, mild to moderately abnormal, and severely abnormal), differences between healthy and injured tissue were apparent on OCT images. Normal images (category 1) had a bilayered structure representative of the epithelium and submucosa. Mild to moderately abnormal images (category 2) had areas of normal and abnormal epithelium. Severely abnormal images (category 3) had complete loss of the epithelium and/or inflammation, with loss of the bilayered structure on OCT.
OCT is a noninvasive imaging modality complementary to colposcopy. It distinguished between normal and abnormal (or injured) tissue and thus holds promise for safety evaluations of candidate microbicides and other vaginal products.
安全性是局部杀菌剂研发中的首要关注点。光学相干断层扫描（OCT）是一种高分辨率、深度横断面成像方式，它与阴道镜检查相结合，用于评估可能预测产品安全性的宫颈阴道上皮变化。
在各种条件下，包括机械损伤和壬苯醇醚 - 9处理，在切除组织和活体中获取猕猴阴道和宫颈组织的OCT及阴道镜图像。
开发了一种评分系统，根据表明完整上皮层和炎症存在与否的形态学特征对OCT图像进行分类和量化。使用三个类别（正常、轻度至中度异常、严重异常），健康组织和受损组织在OCT图像上的差异明显。正常图像（第1类）具有代表上皮和黏膜下层的双层结构。轻度至中度异常图像（第2类）有正常和异常上皮区域。严重异常图像（第3类）上皮完全缺失和/或有炎症，OCT上双层结构消失。
OCT是一种与阴道镜检查互补的非侵入性成像方式。它能区分正常组织和异常（或受损）组织，因此有望用于候选杀菌剂和其他阴道产品的安全性评估。
