Jones F E, DeCosse J J, Condon R E
Ann Surg. 1976 Jul;184(1):74-9. doi: 10.1097/00000658-197607000-00013.
The antimicrobial effect of 20 minutes exposure to 10% povidone-iodine solution and to 5% neomycin-erythromycin solution was evaluated in vitro in 6 suspensions of dog feces. Povidone-iodine eliminated aerobic growth (P less than 0.001) and reduced anaerobes 4.01 +/- 1.06 (P less than 0.02); C. perfringens was the only anaerobic organism grown. Forty unprepared dogs underwent resection of the sigmoid colon and primary anastomosis. Twenty received normal saline and 20 povidone-iodine injected intraluminally immediately before resection. The colon contents of povidone-iodine treated dogs grew only 0.07 +/- 0.07 aerobes and 3.74 +/- 0.49 anaerobes (all Clostridia) (log10/ml colon contents) (P less than 0.001). All povidone-iodine dogs survived 3 weeks with no anastomotic leaks; three controls died from anastomotic leak within the first week (P = 0.12). Reexploration of survivors revealed less perianastomotic reaction in the povidone-iodine group. Twenty minutes exposure to povidone-iodine produced a significant decrease in bacterial counts in vitro and in unprepared sigmoid colon. No adverse effects were demonstrated.
在6份犬粪便悬液中对10%聚维酮碘溶液和5%新霉素-红霉素溶液暴露20分钟的抗菌效果进行了体外评估。聚维酮碘消除了需氧菌生长（P<0.001），并使厌氧菌减少4.01±1.06（P<0.02）；产气荚膜梭菌是唯一生长的厌氧生物。40只未做准备的犬接受了乙状结肠切除术和一期吻合术。20只在切除术前立即向肠腔内注射生理盐水，另外20只注射聚维酮碘。聚维酮碘处理组犬的结肠内容物中仅生长0.07±0.07个需氧菌和3.74±0.49个厌氧菌（均为梭菌属）（每毫升结肠内容物的对数）（P<0.001）。所有聚维酮碘处理组的犬均存活3周，无吻合口漏；3只对照组犬在第一周内死于吻合口漏（P=0.12）。对存活犬进行再次探查发现，聚维酮碘组的吻合口周围反应较轻。聚维酮碘暴露20分钟可使体外和未做准备的乙状结肠中的细菌计数显著降低。未显示出不良反应。
