活动性类风湿关节炎患者滑液中表达FoxP3的CD4+CD25+ T细胞与不同趋化因子受体的积聚。
Accumulation of FoxP3-expressing CD4+CD25+ T cells with distinct chemokine receptors in synovial fluid of patients with active rheumatoid arthritis.
作者信息
Jiao Z, Wang W, Jia R, Li J, You H, Chen L, Wang Y
机构信息
Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, China.
出版信息
Scand J Rheumatol. 2007 Nov-Dec;36(6):428-33. doi: 10.1080/03009740701482800.
OBJECTIVE
To explore the presence and characteristics of FoxP3-expressing CD4+CD25+ regulatory T cells in synovial fluid (SF) of patients with active rheumatoid arthritis (RA).
METHODS
The frequency and chemokine receptors expression profile of FoxP3-expressing CD4+CD25+ regulatory T cells in SF and peripheral blood (PB) from RA patients and PB from healthy controls were investigated by flow cytometry using three- or four-colour intracellular staining.
RESULTS
The frequency of CD4+CD25+ FoxP3+ T cells was increased significantly in SF compared with paired PB from RA patients and PB from healthy controls (p<0.05). However, the frequency in PB from RA patients was significantly lower than in PB from healthy controls (p<0.05). Notably, CD4+CD25+FoxP3+ T cells in SF expressed increased levels of inflammation-related trafficking chemokine receptors, such as CCR4, CCR5, and CXCR4.
CONCLUSION
There is an accumulation of FoxP3-expressing regulatory T cells in RA SF, and such recruitment may be dependent on the distinct chemokine receptors expressed on regulatory T cells.
目的
探讨活动期类风湿关节炎（RA）患者滑液（SF）中表达FoxP3的CD4+CD25+调节性T细胞的存在情况及特征。
方法
采用三色或四色细胞内染色的流式细胞术，研究RA患者SF和外周血（PB）以及健康对照PB中表达FoxP3的CD4+CD25+调节性T细胞的频率和趋化因子受体表达谱。
结果
与RA患者配对的PB以及健康对照的PB相比，SF中CD4+CD25+FoxP3+T细胞的频率显著增加（p<0.05）。然而，RA患者PB中的频率显著低于健康对照的PB（p<0.05）。值得注意的是，SF中的CD4+CD25+FoxP3+T细胞表达炎症相关的迁移趋化因子受体（如CCR4、CCR5和CXCR4）的水平增加。
结论
RA滑液中存在表达FoxP3的调节性T细胞的积聚，这种募集可能依赖于调节性T细胞上表达的独特趋化因子受体。
Zotero 插件