未经治疗的类风湿关节炎患者中 CD4CD25Foxp3 T 细胞的比例和表型变化。
The proportion and phenotypic changes of CD4CD25Foxp3 T cells in patients with untreated rheumatoid arthritis.
机构信息
Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Department of Human Anatomy and Histology and Embryology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, China.
出版信息
BMC Immunol. 2022 Sep 6;23(1):41. doi: 10.1186/s12865-022-00517-0.
OBJECTIVE
CD4CD25Foxp3 regulatory T (Treg) cell-mediated immunosuppression is an essential mechanism of rheumatoid arthritis (RA). However, little is known regarding the specific role of CD4CD25Foxp3 Treg cells in RA. This study aimed to investigate the frequency of circulating CD4CD25Foxp3 Treg cells and their role in RA.
METHODS
Sixty-one untreated RA patients and 40 healthy controls (HCs) were enrolled in this study. The proportion of CD4CD25Foxp3 T cells and CD4CD25Foxp3 Tregs; the levels of CTLA4, GITR, Helios, and ICOS; and the production of IL-17A, IFN-γ, and IL-10 were assessed by flow cytometry. The correlation of CD4CD25Foxp3 T cells and CD4CD25Foxp3 Tregs with the clinical indicators was conducted by Spearman correlation analysis.
RESULTS
The proportion of CD4CD25Foxp3 T cells was elevated in RA and positively correlated with disease activity. CD4CD25Foxp3 T cells expressed less Helios and produced more IFN-γ than conventional Tregs in RA. Additionally, the proportion of CD4CD25Foxp3 T cells was positively correlated with DAS28 score, IgG titer, and anti-CCP titer.
CONCLUSIONS
These data indicate that CD4CD25Foxp3 T cells in RA exhibit several different functional properties from conventional Tregs and are correlated with RA disease activity.
目的
CD4CD25Foxp3 调节性 T（Treg）细胞介导的免疫抑制是类风湿关节炎（RA）的一个重要机制。然而，关于 CD4CD25Foxp3 Treg 细胞在 RA 中的具体作用知之甚少。本研究旨在探讨循环 CD4CD25Foxp3 Treg 细胞的频率及其在 RA 中的作用。
方法
本研究纳入了 61 例未经治疗的 RA 患者和 40 名健康对照者（HCs）。通过流式细胞术评估 CD4CD25Foxp3 T 细胞和 CD4CD25Foxp3 Tregs 的比例；CTLA4、GITR、Helios 和 ICOS 的水平；以及 IL-17A、IFN-γ 和 IL-10 的产生。通过 Spearman 相关分析探讨 CD4CD25Foxp3 T 细胞和 CD4CD25Foxp3 Tregs 与临床指标的相关性。
结果
RA 患者中 CD4CD25Foxp3 T 细胞的比例升高，并与疾病活动度呈正相关。RA 中 CD4CD25Foxp3 T 细胞表达的 Helios 较少，产生的 IFN-γ 多于常规 Tregs。此外，CD4CD25Foxp3 T 细胞的比例与 DAS28 评分、IgG 滴度和抗 CCP 滴度呈正相关。
结论
这些数据表明，RA 中的 CD4CD25Foxp3 T 细胞表现出与常规 Tregs 不同的几种功能特性，与 RA 疾病活动度相关。
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