Shrestha Arina, Rai Shiba Kumar, Basnyat Sheetal Raj, Rai Chandra Kala, Shakya Bikash
Central Department of Microbiology, Tribhuvan University, Kirtipur.
Nepal Med Coll J. 2007 Sep;9(3):166-9.
A total of 200 soil samples taken from different sites and 1,504 stool samples collected from school children (n = 188) and patients (n = 1,316) visiting the health care centres in Kathmandu Valley were included in this study. Soil samples were investigated for the presence of parasitic eggs using sucrose flotation technique. Stool samples were examined by formal-ether concentration and direct smear techniques. The contamination rate of soil with parasitic eggs and larvae was found to be 28.5% (57/ 200). The overall parasitic infection rates in school children and patients were 42.5% and 2.8%, respectively. Four types of parasites were detected from soil samples, of which Ascaris was the most common. Trichuris was most common among school children whereas Ascaris in patients. Females in both study groups had higher prevalence compared with male counterparts with significantly low rate in health care centre visiting patients (p < 0.05).
本研究纳入了从不同地点采集的200份土壤样本，以及从加德满都谷地医疗中心就诊的学童（n = 188）和患者（n = 1316）中收集的1504份粪便样本。采用蔗糖浮选技术对土壤样本进行寄生虫卵检测。粪便样本采用甲醛 - 乙醚浓缩法和直接涂片法进行检查。发现土壤中寄生虫卵和幼虫的污染率为28.5%（57/200）。学童和患者的总体寄生虫感染率分别为42.5%和2.8%。从土壤样本中检测到四种寄生虫，其中蛔虫最为常见。鞭虫在学童中最为常见，而蛔虫在患者中最为常见。两个研究组中的女性患病率均高于男性，在医疗中心就诊的患者中患病率显著较低（p < 0.05）。
