Kim Taesung, Ahn Jae-Hyung, Choi Min-Kyeong, Weon Hang-Yeon, Kim Mi Sun, Seong Chi Nam, Song Hong-Gyu, Ka Jong-Ok
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
J Microbiol Biotechnol. 2007 Nov;17(11):1890-3.
A bacterium, Burkholderia sp. JBA3, which can mineralize the pesticide parathion, was isolated from an agricultural soil. The strain JBA3 hydrolyzed parathion to p-nitrophenol, which was further utilized as the carbon and energy sources. The parathion hydrolase was encoded by a gene on a plasmid that strain JBA3 harbored, and it was cloned into pUC19 as a 3.7-kbp Sau3AI fragment. The ORF2 (ophB) in the cloned fragment encoded the parathion hydrolase composed of 526 amino acids, which was expressed in E. coli DH10B. The ophB gene showed no significant sequence similarity to most of other reported parathion hydrolase genes.
从农业土壤中分离出一种能使农药对硫磷矿化的细菌——伯克霍尔德氏菌JBA3。菌株JBA3将对硫磷水解为对硝基苯酚，后者被进一步用作碳源和能源。对硫磷水解酶由菌株JBA3所携带质粒上的一个基因编码，并作为一个3.7千碱基对的Sau3AI片段克隆到pUC19中。克隆片段中的开放阅读框2（ophB）编码由526个氨基酸组成的对硫磷水解酶，该酶在大肠杆菌DH10B中表达。ophB基因与大多数其他已报道的对硫磷水解酶基因没有显著的序列相似性。
