Wildemann Britt, Burkhardt Nicole, Luebberstedt Marc, Vordemvenne Thomas, Schmidmaier Gerhard
Center for Musculoskeletal Surgery, Charité-Universitätsmedizin Berlin, Germany.
J Orthop Surg Res. 2007 Dec 20;2:27. doi: 10.1186/1749-799X-2-27.
Growth factors are in clinical use to stimulate bone growth and regeneration. BMP-2 is used in long bone and spinal surgery, PDGFbb for the treatment of periodontal defects and children with growth hormone receptor deficiency are treated with IGF-I.Aim of the present study was the comparative analysis of the effect of these growth factors released from a local drug delivery system on cells of the osteogenic lineage at differing differentiation stages.The experiments with the mesenchymal cell line C2C12 revealed a proliferating effect of all three growth factors and a differentiating effect of BMP-2 with a dramatic increase in alkaline phosphatase activity. None of the growth factors stimulated cell migration.Human osteoblast like cells showed similar results with an increase in proliferation after stimulation with IGF-I or PDGFbb. The enzymatic activity of alkaline phosphatase was enhanced only in the cells stimulated with BMP-2. This group showed also more mineralized matrix compared to the other groups.In conclusion, the growth factors IGF-I and PDGFbb delivered with a local drug delivery system stimulated cell proliferation, whereas BMP-2 showed a dramatic effect on differentiation on osteoblast precursor cells and osteoblast like cells.
生长因子在临床中用于刺激骨生长和再生。骨形态发生蛋白-2（BMP-2）用于长骨和脊柱手术，血小板衍生生长因子BB（PDGFbb）用于治疗牙周缺损，生长激素受体缺乏的儿童则用胰岛素样生长因子-I（IGF-I）进行治疗。本研究的目的是比较从局部给药系统释放的这些生长因子对不同分化阶段成骨谱系细胞的影响。对间充质细胞系C2C12进行的实验显示，所有三种生长因子均具有增殖作用，而BMP-2具有分化作用，碱性磷酸酶活性显著增加。没有一种生长因子刺激细胞迁移。人成骨样细胞也显示出类似结果，在用IGF-I或PDGFbb刺激后增殖增加。仅在用BMP-2刺激的细胞中碱性磷酸酶的酶活性增强。与其他组相比，该组还显示出更多矿化基质。总之，通过局部给药系统递送的生长因子IGF-I和PDGFbb刺激细胞增殖，而BMP-2对成骨前体细胞和成骨样细胞的分化具有显著影响。
