Vivas J, Padilla D, Real F, Bravo J, Grasso V, Acosta F
University Institute of Animal Health, IUSA, University of Las Palmas de Gran Canaria, 35416 Arucas, Las Palmas, Spain.
Vet Microbiol. 2008 May 25;129(1-2):150-5. doi: 10.1016/j.vetmic.2007.11.007. Epub 2007 Nov 17.
Hafnia alvei is considered an opportunistic pathogen of animal and humans, affecting a wide range of homeothermic and poikilothermic hosts with different body temperatures. In this work, H. alvei strains isolated from different sources were studied with regard to their capacity to form biofilms under different environmental conditions. Strain, growth phase, temperature and culture media dependent changes of biofilm formation were semiquantitatively monitored using a microtiter plate method. Our study shows that all strains used could form biofilms in vitro, and that biofilm formation increases dramatically during growth at 25 degrees C but not at 37 degrees C, and decreases at both temperatures in presence of glucose. At 16 degrees C only one strain isolated from a lizard was able to form a dense biofilm showing that the ability to form biofilms in this species is regulated by environmental factors and is also strain specific.
蜂房哈夫尼亚菌被认为是一种动物和人类的机会致病菌，可感染多种具有不同体温的恒温动物和变温动物宿主。在本研究中，对从不同来源分离的蜂房哈夫尼亚菌菌株在不同环境条件下形成生物膜的能力进行了研究。使用微量滴定板法对菌株、生长阶段、温度和培养基依赖性生物膜形成变化进行了半定量监测。我们的研究表明，所有使用的菌株都能在体外形成生物膜，并且在25℃生长期间生物膜形成显著增加，但在37℃时不增加，在两种温度下葡萄糖存在时生物膜形成均减少。在16℃时，仅从蜥蜴分离出的一株菌株能够形成致密的生物膜，表明该物种中生物膜形成能力受环境因素调节且具有菌株特异性。
