Afonina Irina, Metcalf Mark, Mills Alan, Mahoney Walt
Nanogen Inc., Bothell, WA 98021, USA.
Diagn Microbiol Infect Dis. 2008 Apr;60(4):429-32. doi: 10.1016/j.diagmicrobio.2007.10.019. Epub 2008 Feb 21.
Rapid and accurate tests capable of distinguishing Bordetella pertussis from milder Bordetella parapertussis infection can aid in treating patients. We evaluated a novel real-time polymerase chain reaction (PCR) method that allows rapid and accurate diagnosis and distinction between B. pertussis and B. parapertussis infections. The method is based on a fluorescent 5'-minor groove-binding molecule hybridization probe that can clearly distinguish between B. pertussis and B. parapertussis by post-PCR melt curve analysis. The reagents worked equally well in several different real-time PCR instruments. The Bordetella detection reagents are combined with internal control components to account for PCR inhibition without compromising assay sensitivity.
能够区分百日咳博德特氏菌与症状较轻的副百日咳博德特氏菌感染的快速准确检测方法有助于治疗患者。我们评估了一种新型实时聚合酶链反应（PCR）方法，该方法可对百日咳博德特氏菌和副百日咳博德特氏菌感染进行快速准确的诊断和区分。该方法基于一种荧光5'-小沟结合分子杂交探针，通过PCR后熔解曲线分析能够清晰区分百日咳博德特氏菌和副百日咳博德特氏菌。这些试剂在几种不同的实时PCR仪器中均能同样出色地发挥作用。博德特氏菌检测试剂与内部对照组件相结合，可在不影响检测灵敏度的情况下解决PCR抑制问题。
