Müller F M, Heininger U, Schnitzler N, Kockelkorn P, Cloot O, Lorenz C, Haase G
Kinderklinik Universitätsklinikum, Aachen, Germany.
Mol Cell Probes. 1998 Aug;12(4):213-7. doi: 10.1006/mcpr.1998.0173.
A recently developed shared-primer polymerase chain reaction (PCR) was investigated, in an ongoing pertussis surveillance study for discrimination of Bordetella parapertussis and Bordetella pertussis organisms, by using specific biotin-labelled oligonucleotide probes. From a total of 132 samples, 83 were positive by the B. parapertussis specific probe, 33 were positive by the B. pertussis specific probe and 16 samples containing Hemophilus influenzae as a negative control were below threshold by both probes. The shared-primer PCR in combination with specific oligonucleotide probes provides a rapid, sensitive and specific molecular diagnostic tool for future surveillance studies. In addition, it may be used to further investigate whether B parapertussis antigens should be added to acellular pertussis vaccines to protect against B. parapertussis infections.
在一项正在进行的百日咳监测研究中，通过使用特异性生物素标记的寡核苷酸探针，对最近开发的共享引物聚合酶链反应（PCR）进行了研究，以鉴别副百日咳博德特氏菌和百日咳博德特氏菌。在总共132个样本中，83个通过副百日咳博德特氏菌特异性探针检测为阳性，33个通过百日咳博德特氏菌特异性探针检测为阳性，16个含有流感嗜血杆菌作为阴性对照的样本通过两种探针检测均低于阈值。共享引物PCR与特异性寡核苷酸探针相结合，为未来的监测研究提供了一种快速、灵敏且特异的分子诊断工具。此外，它可用于进一步研究是否应将副百日咳博德特氏菌抗原添加到无细胞百日咳疫苗中，以预防副百日咳博德特氏菌感染。
