使用孔雀石绿指示染料比色法检测耐多药或广泛耐药结核病
Colorimetric detection of multidrug-resistant or extensively drug-resistant tuberculosis by use of malachite green indicator dye.
作者信息
Farnia Parissa, Masjedi Mohammad Reza, Mohammadi Foroozan, Tabarsei Payam, Farnia Poopak, Mohammadzadeh Ali Reza, Baghei Parvaneh, Varahram Mohammad, Hoffner Sven, Velayati Ali Akbar
机构信息
Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease, Shaheed Bahonar Ave., Darabad, Tehran 19556, PO Box 19575/154, Iran.
出版信息
J Clin Microbiol. 2008 Feb;46(2):796-9. doi: 10.1128/JCM.01435-07. Epub 2007 Dec 19.
Abstract
The malachite green microtube (MGMT) susceptibility assay was performed directly on sputum specimens (n = 80) and indirectly on Mycobacterium tuberculosis clinical isolates (n = 60). The technique is based on the malachite green dye, which changes color in response to M. tuberculosis growth. The MGMT assay is simple and rapid and does not require expensive instruments.
摘要
对80份痰液标本直接进行了孔雀石绿微管（MGMT）药敏试验，并对60株结核分枝杆菌临床分离株间接进行了该试验。该技术基于孔雀石绿染料，其会根据结核分枝杆菌的生长而变色。MGMT试验简单快速，且不需要昂贵的仪器。
相似文献
1
Colorimetric detection of multidrug-resistant or extensively drug-resistant tuberculosis by use of malachite green indicator dye.使用孔雀石绿指示染料比色法检测耐多药或广泛耐药结核病
J Clin Microbiol. 2008 Feb;46(2):796-9. doi: 10.1128/JCM.01435-07. Epub 2007 Dec 19.
2
Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis.多中心实验室验证比色氧化还原指示剂（CRI）检测法快速检测广泛耐药（XDR）结核分枝杆菌。
J Antimicrob Chemother. 2011 Apr;66(4):827-33. doi: 10.1093/jac/dkq527. Epub 2011 Jan 19.
3
Early detection of multidrug- and pre-extensively drug-resistant tuberculosis from smear-positive sputum by direct sequencing.通过直接测序从痰涂片阳性样本中早期检测耐多药和广泛耐药前结核病
BMC Infect Dis. 2017 Apr 24;17(1):300. doi: 10.1186/s12879-017-2409-6.
4
Evaluation of the microscopic observation drug susceptibility assay for the rapid detection of MDR-TB and XDR-TB in China: a prospective multicentre study.评价中国快速检测耐多药结核病和广泛耐药结核病的显微镜观察药物敏感性试验：一项前瞻性多中心研究。
J Antimicrob Chemother. 2015 Feb;70(2):456-62. doi: 10.1093/jac/dku384. Epub 2014 Sep 28.
5
Additional drug resistance in Mycobacterium tuberculosis isolates from resected cavities among patients with multidrug-resistant or extensively drug-resistant pulmonary tuberculosis. 耐多药或广泛耐药肺结核患者切除空洞中分离的结核分枝杆菌分离株的额外耐药性。
Clin Infect Dis. 2012 Mar;54(6):e51-4. doi: 10.1093/cid/cir904. Epub 2011 Dec 23.
6
Evaluation of Direct Colorimetric MTT Assay for Rapid Detection of Rifampicin and Isoniazid Resistance in Mycobacterium tuberculosis.直接比色MTT法用于快速检测结核分枝杆菌对利福平和异烟肼耐药性的评估
PLoS One. 2016 Dec 28;11(12):e0169188. doi: 10.1371/journal.pone.0169188. eCollection 2016.
7
The Colour Test for drug susceptibility testing of Mycobacterium tuberculosis strains.结核分枝杆菌菌株药物敏感性试验的颜色测试。
Int J Tuberc Lung Dis. 2012 Aug;16(8):1113-8. doi: 10.5588/ijtld.11.0609.
8
Evaluation of Nitrate Reductase Assay for Early Detection of Multi and Extensively Drug Resistance Tuberculosis in our Setup.在我们的机构中评估硝酸还原酶测定法用于早期检测多重和广泛耐药结核病的情况。
J Coll Physicians Surg Pak. 2018 Jan;28(1):22-25. doi: 10.29271/jcpsp.2018.01.22.
9
Evaluation of the nitrate reductase assay for rapid detection of extensively drug-resistant tuberculosis.评估硝酸还原酶测定法快速检测广泛耐药结核病。
Int J Tuberc Lung Dis. 2009 Dec;13(12):1542-9.
10
Mycobacterial diversity causing multi- and extensively drug-resistant tuberculosis in Djibouti, Horn of Africa.在非洲之角吉布提导致多重耐药和广泛耐药结核病的分枝杆菌多样性。
Int J Tuberc Lung Dis. 2016 Feb;20(2):150-3. doi: 10.5588/ijtld.15.0268.
引用本文的文献
1
The Effect of Inoculum Size on Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis.接种物大小对分枝杆菌属结核分枝杆菌药物敏感性试验的影响。
Microbiol Spectr. 2023 Jun 15;11(3):e0031923. doi: 10.1128/spectrum.00319-23. Epub 2023 May 22.
2
Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis.多中心评估结晶紫褪色分析（CVDA）快速检测结核分枝杆菌异烟肼和利福平耐药性。
Sci Rep. 2016 Dec 16;6:39050. doi: 10.1038/srep39050.
3
First-Line Anti-Tubercular Drug Resistance of Mycobacterium tuberculosis in IRAN: A Systematic Review.伊朗结核分枝杆菌的一线抗结核药物耐药性：一项系统评价
Front Microbiol. 2016 Jul 28;7:1139. doi: 10.3389/fmicb.2016.01139. eCollection 2016.
4
Evaluation of crystal violet decolorization assay for minimal inhibitory concentration detection of primary antituberculosis drugs against Mycobacterium tuberculosis isolates.用于检测一线抗结核药物对结核分枝杆菌分离株最小抑菌浓度的结晶紫脱色试验评估。
Mem Inst Oswaldo Cruz. 2016 Jun 10;0(7):0. doi: 10.1590/0074-02760160082.
5
Isoniazid and rifampin drug susceptibility testing: application of 2,3,5-triphenyl tetrazolium chloride assay and microscopic-observation drug-susceptibility assay directly on Ziehl-Neelsen smear positive sputum specimens.异烟肼和利福平药敏试验：2,3,5-三苯基氯化四氮唑试验和显微镜观察药敏试验直接应用于萋-尼涂片阳性痰标本
Braz J Infect Dis. 2016 Jan-Feb;20(1):33-40. doi: 10.1016/j.bjid.2015.09.006. Epub 2015 Nov 27.
6
Evaluation of four colourimetric susceptibility tests for the rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates.用于快速检测耐多药结核分枝杆菌分离株的四种比色药敏试验的评估
Mem Inst Oswaldo Cruz. 2015 Aug;110(5):649-54. doi: 10.1590/0074-02760150136. Epub 2015 Jul 24.
7
A new rapid colourimetric method for testing Mycobacterium tuberculosis susceptibility to isoniazid and rifampicin: a crystal violet decolourisation assay.一种用于检测结核分枝杆菌对异烟肼和利福平敏感性的新型快速比色法：结晶紫脱色试验。
Mem Inst Oswaldo Cruz. 2014 Apr;109(2):246-9. doi: 10.1590/0074-0276140297. Epub 2014 Mar 21.
8
Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the malachite green decolourisation assay.使用孔雀石绿脱色试验快速检测耐多药结核分枝杆菌
Mem Inst Oswaldo Cruz. 2013 Dec;108(8):1021-3. doi: 10.1590/0074-0276130697.
9
Evaluation of colorimetric methods using nicotinamide for rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis.利用烟酰胺的比色法快速检测结核分枝杆菌吡嗪酰胺耐药性的评价。
J Clin Microbiol. 2010 Aug;48(8):2729-33. doi: 10.1128/JCM.00311-10. Epub 2010 Jun 16.
10
Comprehensive treatment of extensively drug-resistant tuberculosis.广泛耐药结核病的综合治疗
N Engl J Med. 2008 Aug 7;359(6):563-74. doi: 10.1056/NEJMoa0800106.
本文引用的文献
1
Worldwide emergence of extensively drug-resistant tuberculosis.广泛耐药结核病在全球范围内的出现。
Emerg Infect Dis. 2007 Mar;13(3):380-7. doi: 10.3201/eid1303.061400.
2
Colorimetric redox-indicator methods for the rapid detection of multidrug resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis.用于快速检测结核分枝杆菌多重耐药性的比色氧化还原指示剂方法：系统评价与荟萃分析
J Antimicrob Chemother. 2007 Feb;59(2):175-83. doi: 10.1093/jac/dkl477. Epub 2006 Nov 28.
3
A new rapid and simple colorimetric method to detect pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide.一种使用烟酰胺检测结核分枝杆菌对吡嗪酰胺耐药性的快速简便比色新方法。
J Antimicrob Chemother. 2006 Aug;58(2):327-31. doi: 10.1093/jac/dkl231. Epub 2006 Jun 2.
4
Bacteriological follow-up of pulmonary tuberculosis treatment: a study with a simple colorimetric assay.肺结核治疗的细菌学随访：一项采用简单比色测定法的研究。
Microbes Infect. 2004 Sep;6(11):972-6. doi: 10.1016/j.micinf.2004.04.017.
5
Application of oxidation-reduction assay for monitoring treatment of patients with pulmonary tuberculosis.氧化还原测定法在监测肺结核患者治疗中的应用。
J Clin Microbiol. 2004 Jul;42(7):3324-5. doi: 10.1128/JCM.42.7.3324-3325.2004.
6
Decolorization of malachite green and crystal violet by waterborne pathogenic mycobacteria.水生致病性分枝杆菌对孔雀石绿和结晶紫的脱色作用
Antimicrob Agents Chemother. 2003 Jul;47(7):2323-6. doi: 10.1128/AAC.47.7.2323-2326.2003.
7
Evaluation of a rapid PCR-based epidemiological typing method for routine studies of Mycobacterium tuberculosis.一种基于快速聚合酶链反应的流行病学分型方法用于结核分枝杆菌常规研究的评估。
J Clin Microbiol. 2002 Feb;40(2):712-4. doi: 10.1128/JCM.40.2.712-714.2002.
8
Molecular detection of Mycobacterium tuberculosis: impact on patient care.结核分枝杆菌的分子检测：对患者护理的影响
Clin Chem. 2001 Aug;47(8):1553-8.
9
Kanamycin susceptibility testing of Mycobacterium tuberculosis using Mycobacterium Growth Indicator Tube and a colorimetric method.使用分枝杆菌生长指示管和比色法对结核分枝杆菌进行卡那霉素敏感性测试。
Antimicrob Agents Chemother. 2001 Jun;45(6):1934-6. doi: 10.1128/AAC.45.6.1934-1936.2001.
10
Multicenter evaluation of the mycobacteria growth indicator tube for testing susceptibility of Mycobacterium tuberculosis to first-line drugs.用于检测结核分枝杆菌对一线药物敏感性的分枝杆菌生长指示管的多中心评估。
J Clin Microbiol. 1999 Jan;37(1):45-8. doi: 10.1128/JCM.37.1.45-48.1999.
Zotero 插件