Ruat Caroline, Caillet Catherine, Bidaut Alexandre, Simon James, Osterhaus Albert D M E
Erasmus Medical Center, Rotterdam, The Netherlands.
J Virol. 2008 Mar;82(5):2565-9. doi: 10.1128/JVI.01928-07. Epub 2007 Dec 19.
We investigated the ability of adjuvanted, inactivated split-virion influenza A virus (H5N1) vaccines to protect against infection and demonstrated that the disease exacerbation phenomenon seen with adjuvanted formaldehyde-inactivated respiratory syncytial virus and measles virus investigational vaccines did not occur with these H5N1 vaccines. Macaques were vaccinated twice with or without an aluminum hydroxide or oil-in-water emulsion adjuvanted vaccine. Three months later, animals were challenged with homologous wild-type H5N1. No signs of vaccine-induced disease exacerbation were seen. With either adjuvant, vaccination induced functional and cross-reactive antibodies and protected the lungs and upper respiratory tract. Without an adjuvant, the vaccine provided partial protection. Best results were obtained with the emulsion adjuvant.
我们研究了佐剂化的甲型流感病毒（H5N1）裂解灭活病毒疫苗预防感染的能力，并证明佐剂化的甲醛灭活呼吸道合胞病毒和麻疹病毒研究性疫苗所出现的疾病加重现象在这些H5N1疫苗中并未出现。猕猴分别接种了含或不含氢氧化铝或水包油乳剂佐剂的疫苗，共接种两次。三个月后，用同源野生型H5N1对动物进行攻击。未观察到疫苗诱导的疾病加重迹象。无论使用哪种佐剂，接种疫苗均能诱导产生功能性和交叉反应性抗体，并保护肺部和上呼吸道。无佐剂时，疫苗提供部分保护。水包油乳剂佐剂的效果最佳。
