Caldeira Roberta Lima, Mendonça Cristiane L G F, Goveia Christiane Oliveira, Lenzi Henrique L, Graeff-Teixeira Carlos, Lima Walter S, Mota Ester M, Pecora Iracy Lea, Medeiros Aline Maria Zigiotto de, Carvalho Omar dos Santos
Laboratório de Helmintoses Intestinais, Instituto René Rachou, Fiocruz, Belo Horizonte, MG, 30190-002, Brazil.
Mem Inst Oswaldo Cruz. 2007 Nov;102(7):887-9. doi: 10.1590/s0074-02762007000700018.
Seeking the identification of Angiostrongylus cantonensis as a potential etiological agent of three clinical cases of eosinophilic meningitis, mollusc specimens were collected in the state of Espírito Santo, Brazil. The snails were identified as Sarasinula marginata (45 specimens), Subulina octona (157), Achatina fulica (45) and Bradybaena similaris (23). Larvae obtained were submitted to polymerase chain reaction and restriction fragment length polymorphism diagnosis. Their genetic profile were corresponded to A. cantonensis. Rattus norvegicus experimentally infected with third-stage larvae, developed menigoencephalitis, and parasites became sexually mature in the lungs. Additionally, larvae obtained from A. fulica snails, from São Vicente, state of São Paulo, also showed genetic profiles of this nematode. This is the first record of Brazilian molluscs infected with this nematode species.
为确定广州管圆线虫是否为三例嗜酸性粒细胞性脑膜炎临床病例的潜在病原体，在巴西圣埃斯皮里图州采集了软体动物标本。这些蜗牛被鉴定为缘桑螺（45只标本）、八角土蜗（157只）、非洲大蜗牛（45只）和同型巴蜗牛（23只）。所获幼虫进行了聚合酶链反应和限制性片段长度多态性诊断。其基因图谱与广州管圆线虫相符。用第三期幼虫进行实验性感染的挪威大鼠发生了脑膜脑炎，寄生虫在肺部性成熟。此外，从圣保罗州圣维森特的非洲大蜗牛中获得的幼虫也显示出该线虫的基因图谱。这是巴西软体动物感染该线虫物种的首次记录。
