Gonçalves Antonio José, Carvalho Lucia Helena de, Serdeira Kauê, Nakai Marianne Yumi, Malavasi Tatiana Ramos
Faculdade de Ciências Médicas da Santa Casa de Misericórdia de São Paulo, São Paulo, Brazil.
Sao Paulo Med J. 2007 Sep 6;125(5):289-91. doi: 10.1590/s1516-31802007000500008.
When null, the mu and theta genes of the glutathione S-transferase system (GSTM1 and GSTT1, respectively) are related to malignant tumors affecting the lungs, colon, prostate, bladder and head and neck. In the thyroid, the appearance of cancer has been correlated with deletion of these genes. The aim of this study was to compare the frequencies of these genes in patients with benign and malignant tumors of the thyroid gland.
This was a cross-sectional clinical trial carried out in the Head and Neck Surgery Division, Faculdade de Medicina da Santa Casa de São Paulo.
Samples of thyroid tissue were collected from 32 patients and divided into two groups: benign tumor (A) and malignant tumor (B). After DNA extraction, the genes were amplified using PCR.
The B group presented four cases of positive genotyping for both genes, seven positive for GSTT1 and negative for GSTM1, two negative for GSTT1 and positive for GSTM1, and only one case of double negative. The A group showed 11 cases with positive genotyping for both genes and none with the double negative genotype.
In this study, there was no relationship between the presence of the GSTT1 and GSTM1 genes and the benign and malignant thyroid tumors.
谷胱甘肽S-转移酶系统的μ和θ基因（分别为GSTM1和GSTT1）缺失时，与影响肺、结肠、前列腺、膀胱及头颈部的恶性肿瘤相关。在甲状腺中，癌症的出现与这些基因的缺失有关。本研究的目的是比较甲状腺良性和恶性肿瘤患者中这些基因的频率。
这是一项在圣保罗圣卡塔琳娜医学院头颈外科进行的横断面临床试验。
从32例患者收集甲状腺组织样本，分为两组：良性肿瘤组（A组）和恶性肿瘤组（B组）。提取DNA后，用聚合酶链反应扩增基因。
B组中，两个基因的基因分型均为阳性的有4例，GSTT1阳性而GSTM1阴性的有7例，GSTT1阴性而GSTM1阳性的有2例，仅1例两个基因均为阴性。A组中，两个基因的基因分型均为阳性的有11例，无两个基因均为阴性的基因型。
在本研究中，GSTT1和GSTM1基因的存在与甲状腺良性和恶性肿瘤之间无关联。
