Zhao Yun, Wang Feng-jun, Wang Pei, Chen Yu, Wang Shi-liang
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, the Third Military Medical University, Chongqing, P. R. China.
Zhonghua Shao Shang Za Zhi. 2007 Aug;23(4):253-6.
To investigate the protective effect of recombinant glucagons like peptide-2 (GLP-2) on intestinal mucosa of rats with severe burns.
SD rats of either sex were randomly divided into 4 groups: normal control (N, n = 6), burn control group (C, n = 6), recombinant GLP-2 group (Gr, n = 6, with subcutaneous injection of 100 nmol x kg(-1) x d(-1) recombinant GLP-2 at 4 post-burn hours (PBH) and synthesized GLP-2 group (G, n = 6, with subcutaneous injection of 100 nmol x kg(-1) x d(-1) synthesized GLP-2 at 4 PBH). Except the normal control group, all animals in the other groups received a 30% TBSA third degree burns, the rats were sacrificed on 7 postburn days (PBD) and the following indexes were determined: pathological examination of intestinal mucosa, mucosa permeability of intestinal mucosa, the ratio of mucosa wet weight and bowel mass or carcase weight, and the protein content of intestinal mucosa.
Compared with that in burn group [(0.350 +/- 0.040) mg/ml], the mucosa permeability significantly decreased in Gr (0.250 +/- 0.026) mg/ml and G (0.243 +/- 0.008) mg/ml groups, while the ratio of mucosa wet weight and carcase weight, the protein content of intestinal mucosa were significantly increased. In addition, the content of intestinal mucosal protein in Gr group [(57.9 +/- 2.8) mg/g wet weight] was higher than that in G group [(48.9 +/- 4.1) mg/g wet weight]. In contrast to normal controls, the villi of intestinal mucosa in rats on 7 PBD were obviously shortened and exfoliated, with deranged disposition and thinned basal membrane. No obvious difference of the injury was observed between Gr and G groups, and the injury was milder when compared with burn group.
Recombinant GLP-2, as well as synthesized GLP-2, exhibits obvious protective effect on intestinal mucosa in alleviating injury to intestinal mucosa in burn rats.
探讨重组胰高血糖素样肽-2（GLP-2）对严重烧伤大鼠肠黏膜的保护作用。
将雌雄不限的SD大鼠随机分为4组：正常对照组（N，n = 6）、烧伤对照组（C，n = 6）、重组GLP-2组（Gr，n = 6，于烧伤后4小时（PBH）皮下注射100 nmol·kg⁻¹·d⁻¹重组GLP-2）和合成GLP-2组（G，n = 6，于烧伤后4小时皮下注射100 nmol·kg⁻¹·d⁻¹合成GLP-2）。除正常对照组外，其他组动物均给予30%体表面积的Ⅲ度烧伤，于烧伤后7天（PBD）处死大鼠，测定以下指标：肠黏膜病理检查、肠黏膜通透性、黏膜湿重与肠重或体重之比以及肠黏膜蛋白含量。
与烧伤组[（0.350±0.040）mg/ml]相比，Gr组（0.250±0.026）mg/ml和G组（0.243±0.008）mg/ml的黏膜通透性显著降低，而黏膜湿重与体重之比、肠黏膜蛋白含量显著增加。此外，Gr组[（57.9±2.8）mg/g湿重]的肠黏膜蛋白含量高于G组[（48.9±4.1）mg/g湿重]。与正常对照组相比，烧伤后7天大鼠的肠黏膜绒毛明显缩短、脱落，排列紊乱，基底膜变薄。Gr组和G组之间损伤无明显差异，且与烧伤组相比损伤较轻。
重组GLP-2以及合成GLP-2对肠黏膜均具有明显的保护作用，可减轻烧伤大鼠肠黏膜的损伤。
