Li Li, Zhang Xiaopeng, Zhang Wenzhong, Song Yan
Institute for Nutrition and Food Safety, Chinese Centre for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2007 Sep;36(5):564-7.
To study the effects of lactational exposure to soy isoflavones (SIF) on ovary development in neonate rats.
Virgin Sprague-Dawley female and male rats were fed with soy and alfalfa free diets (SAFD). The pregnant rats were randomly divided into six groups with seven animals in each group. Dams in different treatment groups received 0, 10, 50, 100, 150 and 200mg/kg bw of SIF from postnatal day 5 to 10 (PND5-10) by gavage, respectively. On PND11, female litters were killed and ovaries were removed for pathological evaluation and detection of estrogen receptor (ER), andron receptor (AR), progesterone receptor (PR), proliferating cell nuclear antigen (PCNA) protein expression by immunohistochemistry as well as ER, AR, PR mRNA expression by RT-PCR.
The ratios of ovary weight to body weight increased significantly in 100, 150 and 200 mg/kg bw groups than that in control group. And the ovary follicles developed more quickly in offsprings exposed to SIF. In 150 and 200 mg/kg bw groups, ER, PCNA protein and ER mRNA expression in ovaries increased significantly, while PR mRNA expression decreased significantly. Other receptor expressions had no significant difference from the control group.
Lactational exposure to soy isoflavones could induce adverse effects on ovary development in neonate rats, which mechanisms may, at least, partically involved with modification of mRNA transcription for ER and PR.
研究哺乳期暴露于大豆异黄酮（SIF）对新生大鼠卵巢发育的影响。
将未孕的斯普拉格-道利（Sprague-Dawley）雌雄大鼠喂以不含大豆和苜蓿的饲料（SAFD）。将怀孕大鼠随机分为六组，每组七只。不同处理组的母鼠在出生后第5天至第10天（PND5 - 10）分别通过灌胃给予0、10、50、100、150和200mg/kg体重的SIF。在PND11时，处死雌性幼崽并取出卵巢进行病理评估，通过免疫组织化学检测雌激素受体（ER）、雄激素受体（AR）、孕激素受体（PR）、增殖细胞核抗原（PCNA）蛋白表达，以及通过逆转录聚合酶链反应（RT-PCR）检测ER、AR、PR mRNA表达。
100、150和200mg/kg体重组的卵巢重量与体重之比显著高于对照组。并且暴露于SIF的后代卵巢卵泡发育更快。在150和200mg/kg体重组中，卵巢中ER、PCNA蛋白和ER mRNA表达显著增加，而PR mRNA表达显著降低。其他受体表达与对照组无显著差异。
哺乳期暴露于大豆异黄酮可对新生大鼠卵巢发育产生不良影响，其机制可能至少部分与ER和PR的mRNA转录改变有关。
