Chen Shi-wei, Zhang Hong-min, Zhang Li-shi, Feng Xiao-fan
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Sep;37(5):717-20.
To assess the effects of soy isoflavone (SIF) on improving insulin reistance (IR) status in IR rats induced by high-fat and high-sugar diet and explore the possible mechanisms.
IR rats were randomly divided into four groups according to their insulin resistance indices (IRI). The rats in one model control group and three SIF groups were fed via gavage with water solutions containing SIF at doses of 0 mg/ kg x bw, 50 mg/kg x bw, 150 mg/kg x bw, and 450 mg/kg x bw, respectively. After one month, fasting glucose, fasting insulin, resistin in serum, and resistin mRNA in adipocyte around kidney were detected by enzymologic method, radioimmunoassay, enzyme linked immunosorbent assay, and real time RT-PCR, respectively.
Comparison between the model control group and the other groups revealed that serum resistin and resistin mRNA expression levels were lower in the 450 mg/kg x bw group, that insulin and IRI levels were lower in the 150 mg/ kg x bw group and 450 mg/kg x bw group, and that no differences in plasma glucose levels existed among the 4 groups. A positive correlation between IRI and serum resistin level (r = 0.355, P < 0.05) was observed.
These results suggest that soy isoflavone may down-regulate resistin mRNA expression, decrease serum resistin level and enhance insulin sensitivity.
评估大豆异黄酮（SIF）对改善高脂高糖饮食诱导的胰岛素抵抗（IR）大鼠胰岛素抵抗状态的影响，并探讨其可能机制。
根据胰岛素抵抗指数（IRI）将IR大鼠随机分为四组。模型对照组和三个SIF组大鼠分别经口灌胃给予含SIF剂量为0mg/kg体重、50mg/kg体重、150mg/kg体重和450mg/kg体重的水溶液。1个月后，分别采用酶学法、放射免疫法、酶联免疫吸附测定法和实时RT-PCR检测空腹血糖、空腹胰岛素、血清抵抗素及肾周脂肪细胞抵抗素mRNA。
模型对照组与其他组比较，450mg/kg体重组血清抵抗素及抵抗素mRNA表达水平较低，150mg/kg体重组和450mg/kg体重组胰岛素及IRI水平较低，4组间血糖水平无差异。观察到IRI与血清抵抗素水平呈正相关（r = 0.355，P < 0.05）。
这些结果表明，大豆异黄酮可能下调抵抗素mRNA表达，降低血清抵抗素水平，增强胰岛素敏感性。
