[芬太尼与乳化异氟烷的催眠相互作用]

[Fentanyl hypnotic interaction with emulsified isoflurane].

作者信息

Yang Jing-wen, Yi Ming-liang, Chai Yun-fei, Zhang Wen-sheng, Liu Jin

机构信息

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Nov;38(6):973-6.

PMID:18095599

Abstract

OBJECTIVES

To explore the effect of fentanyl on 50% effective dose (ED50) of emulsified isoflurane for hypnosis and to define the type of interaction between fentanyl and emulsified isoflurane during rat hypnosis.

METHODS

125 male adult Sprague-Dawley rats (240-300 g) were divided into 5 groups depending on the drugs administered via the tail vein. The up-and-down sequential allocation technique was used to determine ED50 of fentanyl in the loss of righting reflex. According to above experiment results, the up-and-down sequential allocation technique was also used to determine ED50 of emulsified isoflurane combined with different fentanyl dose of 7.2 microg/kg (1/4 ED50), 14.3 microg/kg (1/2 ED50) or 21.5 microg/kg (3/4 ED50), respectively, and also without the fentanyl in the loss of righting reflex. Fentanyl was administered intravenously for 20 s followed by emulsified isoflurane for 10 s. The interval between the two was 1.5 min in groups administered with both. ED50 was calculated by using Dixon-Mood method. The isobolographic and algebraic analyses were used to define the type of interaction between fentanyl and emulsified isoflurane.

RESULTS

The body weight and sex of rats was not significantly different between the 5 groups. ED50 was (28.7 +/- 2.1) microg/kg (26.5-30.8 microg/kg) in the fentanyl group, (0.525 +/- 0.032) mL/kg in the fentanyl 7.2 microg/kg +/- isoflurane group, (0.108 +/- 0.019) mL/kg in the fentanyl 14.3 microg/kg+isoflurane group, (0.075 +/- 0.011) mL/kg in the fentanyl 21.5 microg/kg+isoflurane group and (0.670 +/- 0.054) mL/kg in the isoflurane group. The isobolographic analysis indicated that ED50 of isoflurane decreased progressively in a non-linear fashion in the presence of increasing dose of fentanyl. The isobolographic and algebraic analyses demonstrated that the combination use of fentanyl and emulsified isoflurane could produce a synergistic effect on hypnosis.

CONCLUSIONS

Fentanyl enhances the hypnotic effect induced by emulsified isoflurane. The administration of associating fentanyl with emulsified isoflurane produces a synergistic effect on rat hypnosis.

摘要

目的

探讨芬太尼对乳化异氟烷催眠半数有效剂量（ED50）的影响，并确定大鼠催眠过程中芬太尼与乳化异氟烷之间的相互作用类型。

方法

125只成年雄性Sprague-Dawley大鼠（240 - 300 g）根据经尾静脉给药情况分为5组。采用序贯法确定芬太尼使翻正反射消失的ED50。根据上述实验结果，序贯法还用于分别确定乳化异氟烷与7.2 μg/kg（1/4 ED50）、14.3 μg/kg（1/2 ED50）或21.5 μg/kg（3/4 ED50）不同剂量芬太尼联合应用以及单独应用乳化异氟烷使翻正反射消失的ED50。静脉注射芬太尼20 s，随后静脉注射乳化异氟烷10 s。联合用药组两者间隔1.5 min。采用Dixon-Mood法计算ED50。采用等效应线图和代数分析确定芬太尼与乳化异氟烷之间的相互作用类型。

结果

5组大鼠的体重和性别无显著差异。芬太尼组ED50为（28.7±2.1）μg/kg（26.5 - 30.8 μg/kg），芬太尼7.2 μg/kg + 异氟烷组为（0.525±0.032）mL/kg，芬太尼14.3 μg/kg + 异氟烷组为（0.108±0.019）mL/kg，芬太尼21.5 μg/kg + 异氟烷组为（0.075±0.011）mL/kg，异氟烷组为（0.670±0.054）mL/kg。等效应线图分析表明，随着芬太尼剂量增加，异氟烷的ED50呈非线性逐渐降低。等效应线图和代数分析表明，芬太尼与乳化异氟烷联合应用对催眠可产生协同作用。