与年龄相关的晶状体纤维致密化会影响兔晶状体的结构和光学特性。

Age-related compaction of lens fibers affects the structure and optical properties of rabbit lenses.

作者信息

Al-Khudari Samer, Donohue Sean T, Al-Ghoul Walid M, Al-Ghoul Kristin J

机构信息

Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL, USA.

出版信息

BMC Ophthalmol. 2007 Dec 20;7:19. doi: 10.1186/1471-2415-7-19.

DOI:10.1186/1471-2415-7-19

PMID:18096063

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2249566/

Abstract

BACKGROUND

The goal of this investigation was to correlate particular age-related structural changes (compaction) to the amount of scatter in rabbit lenses and to determine if significant fiber compaction occurred in the nuclear and inner cortical regions.

METHODS

New Zealand White rabbits at 16-20 months old (adult; n = 10) and at 3.5-4 years old (aged; n = 10) were utilized for this study. Immediately after euthanising, scatter was assessed in fresh lenses by low power helium-neon laser scan analysis. Scatter data was analyzed both for whole lenses and regionally, to facilitate correlation with morphometric data. After functional analysis, lenses were fixed and processed for scanning electron microcopy (SEM; right eyes) and light microscopy (LM; left eyes). Morphometric analysis of SEM images was utilized to evaluate compaction of nuclear fibers. Similarly, measurements from LM images were used to assess compaction of inner cortical fibers.

RESULTS

Scatter was significantly greater in aged lenses as compared to adult lenses in all regions analyzed, however the difference in the mean was slightly more pronounced in the inner cortical region. The anterior and posterior elliptical angles at 1 mm (inner fetal nucleus) were significantly decreased in aged vs. adult lenses (anterior, p = 0.040; posterior, p = 0.036). However, the average elliptical angles at 2.5 mm (outer fetal nucleus) were not significantly different in adult and aged lenses since all lenses examined had comparable angles to inner fetal fibers of aged lenses, i.e. they were all compacted. In cortical fibers, measures of average cross-sectional fiber area were significantly different at diameters of both 6 and 7 mm as a function of age (p = 0.011 and p = 0.005, respectively). Accordingly, the estimated fiber volume was significantly decreased in aged as compared to adult lenses at both 6 mm diameter (p = 0.016) and 7 mm diameter (p = 0.010).

CONCLUSION

Morphometric data indicates that inner cortical fibers undergo a greater degree of age-related compaction than nuclear fibers. Increased scatter appears to be only tentatively correlated with regions of fiber compaction, suggesting that it is simply one of an array of factors that contribute to the overall decreased transparency in aged rabbit lenses.

摘要

背景

本研究的目的是将特定的年龄相关结构变化（压实）与兔晶状体中的散射量相关联，并确定在核区和内皮质区是否发生了显著的纤维压实。

方法

本研究使用了16 - 20月龄（成年；n = 10）和3.5 - 4岁（老龄；n = 10）的新西兰白兔。安乐死后，立即通过低功率氦氖激光扫描分析对新鲜晶状体中的散射进行评估。对整个晶状体和区域散射数据进行分析，以便与形态测量数据相关联。功能分析后，将晶状体固定并进行扫描电子显微镜（SEM；右眼）和光学显微镜（LM；左眼）检查。利用SEM图像的形态测量分析来评估核纤维的压实情况。同样，利用LM图像的测量结果来评估内皮质纤维的压实情况。

结果

在所有分析区域中，老龄晶状体的散射均显著高于成年晶状体，然而，内皮质区的平均差异更为明显。与成年晶状体相比，老龄晶状体在1 mm（内胎儿核）处的前后椭圆角显著减小（前，p = 0.040；后，p = 0.036）。然而，2.5 mm（外胎儿核）处的平均椭圆角在成年和老龄晶状体中无显著差异，因为所有检查的晶状体与老龄晶状体的内胎儿纤维角度相当，即它们都已压实。在皮质纤维中，6 mm和7 mm直径处的平均横截面纤维面积测量值随年龄有显著差异（分别为p = 0.011和p = 0.005）。因此，与成年晶状体相比，老龄晶状体在6 mm直径（p = 0.016）和7 mm直径（p = 0.010）处的估计纤维体积均显著减小。