Castro F F, Schmitz-Schumann M, Rother U, Kirschfink M
Institute of Immunology, University of Heidelberg, FRG.
Int Arch Allergy Appl Immunol. 1991;96(4):305-10. doi: 10.1159/000235513.
Among ten different allergens, house-dust extract proved to be the most potent complement activator. It was therefore chosen to investigate the susceptibility of complement in the serum and bronchoalveolar lavage fluid of patients with extrinsic asthma and control persons. Complement activation in serum was assessed by the appearance of C3d as well as the activation-specific protein-protein complexes C1rs-C1inhibitor (classical pathway) and C3b(Bb)P (alternative pathway). Complement was activated via both the classical and the alternative pathway in a dose- and time-dependent manner. In contrast to earlier observations, however, complement was less affected in the serum of asthmatics than in the serum of normal individuals. Differences were restricted to alternative-pathway activation, probably due to preactivation and/or a significantly higher serum concentration of the regulatory protein factor H in asthmatic patients. In vitro generation of C3a in bronchoalveolar lavage fluid could not be achieved, although the presence of alternative pathway proteins C3, B and D was demonstrated.
在十种不同的变应原中，屋尘提取物被证明是最有效的补体激活剂。因此，选择它来研究外源性哮喘患者和对照者血清及支气管肺泡灌洗液中补体的敏感性。通过C3d的出现以及激活特异性蛋白-蛋白复合物C1rs-C1抑制剂（经典途径）和C3b(Bb)P（替代途径）来评估血清中的补体激活。补体通过经典途径和替代途径以剂量和时间依赖性方式被激活。然而，与早期观察结果相反，哮喘患者血清中的补体受影响程度低于正常个体血清。差异仅限于替代途径激活，这可能是由于哮喘患者中调节蛋白因子H的预激活和/或血清浓度显著升高。尽管在支气管肺泡灌洗液中证实存在替代途径蛋白C3、B和D，但无法实现C3a的体外生成。
