Tanimoto K, Le T, Zhu L, Chen J, Featherstone J D B, Li W, DenBesten P
Department of Orofacial Sciences, University of California, San Francisco, 513 Parnasuss Avenue, San Francisco, CA 94143, USA.
J Dent Res. 2008 Jan;87(1):39-44. doi: 10.1177/154405910808700106.
Fluorosed enamel is more porous and less mineralized, possibly related to altered amelogenin-modulated crystal growth. The purpose of this study was to examine the role of fluoride in interactions between amelogenin and apatite crystals. Recombinant human amelogenin (rh174) was bound to carbonated hydroxyapatite containing various amounts of fluoride, and analyzed by protein assay, SDS PAGE, and AFM. Interactions between rh174 and fluoride were assayed by isothermal titration calorimetry (ITC). The initial binding rate of rh174, as well as total amount of rh174 bound to fluoride-containing carbonated hydroxyapatite, was greater than that in the control carbonated hydroxyapatite. Fluoride in solution at physiologic (5.3 micromolar, or 0.1 ppm) concentrations showed no significant effect on binding, but higher fluoride levels significantly decreased protein binding. ITC showed no interactions between fluoride and rh174. These results suggest that fluoride incorporation into the crystal lattice alters the crystal surface to enhance amelogenin binding, with no direct interactions between fluoride and amelogenin.
氟斑牙釉质孔隙更多且矿化程度更低，这可能与釉原蛋白调节的晶体生长改变有关。本研究的目的是研究氟在釉原蛋白与磷灰石晶体相互作用中的作用。将重组人釉原蛋白（rh174）与含有不同氟含量的碳酸羟基磷灰石结合，并通过蛋白质测定、SDS-PAGE和原子力显微镜进行分析。通过等温滴定量热法（ITC）测定rh174与氟之间的相互作用。rh174的初始结合速率以及与含氟碳酸羟基磷灰石结合的rh174总量均高于对照碳酸羟基磷灰石。生理浓度（5.3微摩尔，或0.1 ppm）的溶液中的氟对结合没有显著影响，但较高的氟水平显著降低了蛋白质结合。ITC显示氟与rh174之间没有相互作用。这些结果表明，氟掺入晶格会改变晶体表面以增强釉原蛋白结合，而氟与釉原蛋白之间没有直接相互作用。
