变形链球菌中一种假定的糖激酶SMU.573的初步X射线晶体学分析。
Preliminary X-ray crystallographic analysis of SMU.573, a putative sugar kinase from Streptococcus mutans.
作者信息
Zhou Yan-Feng, Li Lan-Fen, Yang Cheng, Liang Yu-He, Su Xiao-Dong
机构信息
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):47-9. doi: 10.1107/S1744309107065645. Epub 2007 Dec 20.
Abstract
SMU.573 from Streptococcus mutans is a structurally and functionally uncharacterized protein that was selected for structural biology studies. Native and SeMet-labelled proteins were expressed with an N-His tag in Escherichia coli BL21 (DE3) and purified by Ni2+-chelating and size-exclusion chromatography. Crystals of the SeMet-labelled protein were obtained by the hanging-drop vapour-diffusion method and a 2.5 A resolution diffraction data set was collected using an in-house chromium radiation source. The crystals belong to space group I4, with unit-cell parameters a = b = 96.53, c = 56.26 A, alpha = beta = gamma = 90 degrees.
摘要
变形链球菌来源的SMU.573是一种结构和功能均未明确的蛋白质，被选用于结构生物学研究。天然蛋白和硒代甲硫氨酸标记的蛋白在大肠杆菌BL21(DE3)中以N端组氨酸标签进行表达，并通过镍离子螯合和尺寸排阻色谱法进行纯化。硒代甲硫氨酸标记的蛋白晶体通过悬滴气相扩散法获得，并使用内部铬辐射源收集了分辨率为2.5埃的衍射数据集。晶体属于空间群I4，晶胞参数为a = b = 96.53，c = 56.26埃，α = β = γ = 90°。
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