Zhou Xin-Rong, Yuan Hui-Ping, Qu Wei, Ma Chun-Yang, Li Hong-Yi, Wang Ya
Department of Ophthalmology, Harbin Medical University, Second Affiliated Hospital, Harbin, China.
Ophthalmologica. 2008;222(1):6-10. doi: 10.1159/000109271. Epub 2007 Dec 19.
To observe the influence on the retinal ganglion cells induced by 2,450-MHz microwave. The radiant intensities were 10, 30 and 60 mW x cm(-2).
Retinal ganglion cells were cultured in vitro and divided into 4 groups, of which 3 were exposed to different intensities of microwaves and 1 was the control group. The morphological variation of cells was observed by invert microscope. The survival rate was assessed by trypan blue. Annexin V-PI 2-color flow cytometry was used to detect the percentage of earlier apoptosis cells after radiation.
The changes in the morphology of all cells were observed after radiation. The cell survival rates were reduced and the earlier apoptosis increased with growing microwave intensity.
The 2,450-MHz microwave will cause damage to retinal ganglion cells in a dose-dependent manner.
观察2450兆赫微波对视网膜神经节细胞的影响。辐射强度分别为10、30和60毫瓦×厘米（-2）。
体外培养视网膜神经节细胞，分为4组，其中3组暴露于不同强度的微波下，1组为对照组。通过倒置显微镜观察细胞的形态变化。用台盼蓝评估细胞存活率。采用Annexin V-PI双色流式细胞术检测辐射后早期凋亡细胞的百分比。
辐射后观察到所有细胞的形态均发生变化。随着微波强度的增加，细胞存活率降低，早期凋亡增加。
2450兆赫微波将以剂量依赖的方式对视网膜神经节细胞造成损伤。
