Wei Ai-min, Yang Xue-sen, Wang Yuan, He Gen-lin, Zhou Zhou, Zhang Guang-bin, Yu Zheng-ping
Key Laboratory of Medical Protection for Electromagnetic Radiation Ministry of Education, Third Military Medical University, Chongqing 400038, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012 Mar;30(3):172-7.
To investigate the injury effects of microwave on the visual performance and the apoptosis of retinal ganglion cells (RGCs) in rats and the relationship between the impaired visual performance and RGCs apoptosis induced by microwave.
The visual performance of rats was observed by Electroretinogram (ERG) and Flash visual evoked potentials (F-VEP). The apoptosis of RGCs in vivo and in vitro was detected by TUNEL assay and Hoechst staining.
Microwave exposure had no influence on ERG-a wave. The amplitude of ERG-b wave decreased significantly on the 3rd day and 7th day after microwave exposure (P < 0.01).The latency of ERG-b wave shortened significantly only at 3rd day after microwave exposure (P < 0.01). The latency of F-VEP extended markedly on the 3rd day after exposure (P < 0.05) and recovered on the 7th day after microwave exposure. The amplitude of F-VEP decreased significantly in exposure group, as compared with sham-exposure group, on the 3rd day and 7th day after microwave exposure (P < 0.05). After microwave exposure for 12 h, the apoptotic rate of RGCs in rat increased from 2.85% to 6.73%, and on the 7th day after exposure, the apoptotic rate of RGCs remained 8.93% (P < 0.05). The apoptotic rate of cultured RGCs increased from 8.42% to 13.91% at 6 hour (P < 0.05) and to 24.14% at 24 hour (P < 0.01) after microwave exposure (P < 0.05 or P < 0.01).
Microwave exposure can injure the visual performance of rats, and the apoptosis of RGCs induced microwave may be one of the main pathological mechanisms.
探讨微波对大鼠视觉功能及视网膜神经节细胞（RGCs）凋亡的损伤作用，以及微波所致视觉功能损害与RGCs凋亡之间的关系。
采用视网膜电图（ERG）和闪光视觉诱发电位（F-VEP）观察大鼠的视觉功能。通过TUNEL法和Hoechst染色检测体内及体外RGCs的凋亡情况。
微波照射对ERG-a波无影响。微波照射后第3天和第7天，ERG-b波振幅显著降低（P<0.01）。仅在微波照射后第3天，ERG-b波潜伏期显著缩短（P<0.01）。照射后第3天，F-VEP潜伏期明显延长（P<0.05），并在微波照射后第7天恢复。与假照射组相比，微波照射组在照射后第3天和第7天F-VEP振幅显著降低（P<0.05）。微波照射12小时后，大鼠RGCs凋亡率从2.85%升至6.73%，照射后第7天，RGCs凋亡率仍为8.93%（P<0.05）。微波照射后6小时，培养的RGCs凋亡率从8.42%升至13.91%（P<0.05），24小时时升至24.14%（P<0.01）。
微波照射可损伤大鼠的视觉功能，微波诱导的RGCs凋亡可能是主要病理机制之一。
