使用停流双波长分光光度计对在多种条件下生长的大肠杆菌膜结合细胞色素进行动力学表征。
Kinetic characterization of the membrane-bound cytochromes of Escherichia coli grown under a variety of conditions by using a stopped-flow dual-wavelength spectrophotometer.
作者信息
Haddock B A, Downie J A, Garland P B
出版信息
Biochem J. 1976 Feb 15;154(2):285-94. doi: 10.1042/bj1540285.
Abstract
A study was made of the rapid oxidation kinetics of the cytochromes of Escherichia coli. The b-type cytochromes were kinetically heterogeneous, with one species (presumably cytochrome o) oxidized so rapidly that it could fully support observed oxidation rates. Cytochrome d but not cytochrome a1 was also kinetically competent to support respiration. However, in cells grown anaerobically in the presence of NO3-, cytochrome d exhibited slow oxidation kinetics and a red-shift in its reduced-minus-oxidized difference spectrum.
摘要
对大肠杆菌细胞色素的快速氧化动力学进行了研究。b型细胞色素在动力学上具有异质性，其中一种（可能是细胞色素o）氧化速度极快，以至于它能够完全支持观察到的氧化速率。细胞色素d而非细胞色素a1在动力学上也能够支持呼吸作用。然而，在存在NO3-的情况下厌氧生长的细胞中，细胞色素d表现出缓慢的氧化动力学，并且其还原态减去氧化态差光谱发生红移。
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