Hackett N R, Bragg P D
J Bacteriol. 1983 May;154(2):708-18. doi: 10.1128/jb.154.2.708-718.1983.
Redox titration has been coupled to spectroscopic techniques, enzyme fractionation, and the use of mutants to examine the cytochrome composition of the membranes from cells grown aerobically and anaerobically with nitrate. A combination of techniques was found to be necessary to resolve the cytochromes. At least six b-type cytochromes were present. Besides cytochromes bfdh and bnr, components of the formate dehydrogenase-nitrate reductase pathway, cytochromes b556, b555, b562, and o, characteristic of aerobic respiratory pathways, were present. The midpoint oxidation-reduction potentials of the aerobic b-type cytochromes suggested that the sequence of electron transfer is: cytochrome b556 leads to b555 leads to b562 leads to O2.
氧化还原滴定已与光谱技术、酶分级分离以及使用突变体相结合,以研究在有氧和以硝酸盐为厌氧条件下生长的细胞内膜的细胞色素组成。发现需要结合多种技术来解析细胞色素。至少存在六种b型细胞色素。除了甲酸脱氢酶 - 硝酸盐还原酶途径的组分细胞色素bfdh和bnr外,还存在有氧呼吸途径特有的细胞色素b556、b555、b562和o。有氧b型细胞色素的中点氧化还原电位表明电子传递顺序为:细胞色素b556导致b555导致b562导致O2。