Osawa Hiroyuki, Ohnishi Hirohide, Kita Hiroto, Igari Toru, Hayashi Yukiko, Iwamori Masao, Kawakami Masanobu, Sugano Kentaro
Department of Internal Medicine, Jichi Medical University, Tochigi, Japan.
J Clin Gastroenterol. 2008 Jan;42(1):23-8. doi: 10.1097/01.mcg.0000225663.61297.03.
Sulfatides highly expressed in the normal gastric mucosa play important roles in gastric mucosal protection. However, it is unknown whether the sulfatides expression changes in chronic gastritis.
Sulfatides expression levels were examined with immunohistochemical staining in 77 specimens obtained from resected human stomachs, followed by the measurement of optical density of the staining under digital microscopy. For mRNA expression analysis of GalCer (galactosylceramide)-sulfotransferase and arylsulfatase A using a quantitative real time reverse transcription-polymerase chain reaction, 64 biopsy specimens were endoscopically taken from the gastric corpus of out patients. Mucosal changes were scored under microscopic observations according to the updated Sydney System classification.
The sulfatides expression decreased along with the progression of mucosal atrophy and lymphocytes infiltration, and was barely observed in intestinal metaplasia. The mRNA expression of GalCer-sulfotransferase, a biosynthetic enzyme for sulfatides, also decreased along with the progression of mucosal atrophy and lymphocytes infiltration. In contrast, mRNA expression of arylsulfatase A, which degrades sulfatides, was not altered.
Expression of sulfatides and GalCer-sulfotransferase in the gastric mucosa of chronic gastritis is selectively reduced according to the mucosal atrophy and inflammation. Decreased expression of sulfatides may attribute to the deterioration of mucosal protection in chronic gastritis.
正常胃黏膜中高表达的硫脂在胃黏膜保护中发挥重要作用。然而，慢性胃炎中硫脂表达是否发生变化尚不清楚。
采用免疫组织化学染色法检测77例人胃切除标本中硫脂的表达水平，然后在数字显微镜下测量染色的光密度。为了使用定量实时逆转录-聚合酶链反应分析半乳糖基神经酰胺（GalCer）-硫酸转移酶和芳基硫酸酯酶A的mRNA表达，从门诊患者的胃体部内镜取64例活检标本。根据更新的悉尼系统分类，在显微镜下观察对黏膜变化进行评分。
硫脂表达随着黏膜萎缩和淋巴细胞浸润的进展而降低，在肠化生中几乎未观察到。硫脂生物合成酶GalCer-硫酸转移酶的mRNA表达也随着黏膜萎缩和淋巴细胞浸润的进展而降低。相比之下，降解硫脂的芳基硫酸酯酶A的mRNA表达未改变。
慢性胃炎胃黏膜中硫脂和GalCer-硫酸转移酶的表达根据黏膜萎缩和炎症情况选择性降低。硫脂表达降低可能导致慢性胃炎中黏膜保护功能恶化。
