17β-雌二醇对雄性雏鸡原始生殖细胞迁移分布的影响。
Effects of 17beta-estradiol on distribution of primordial germ cell migration in male chicks.
作者信息
Jin Xiu-Mei, Zhang Yi-Xiang, Li Zan-Dong
机构信息
College of Biological Sciences, China Agricultural University, Yuan-Ming Yuan West Road 2, Beijing, China.
出版信息
Asian J Androl. 2008 Mar;10(2):243-8. doi: 10.1111/j.1745-7262.2008.00330.x. Epub 2007 Dec 20.
AIM
To assess whether exogenous estradiol has any effect on migration of primordial germ cells (PGCs) in the chick.
METHODS
Fertilized eggs were treated with 17beta-estradiol (E(2)) (80 microg/egg) at stage X (day 0 of incubation), stages 8-10 (incubation 30 h) and 13-15 (incubation 55 h). Controls received vehicle (emulsion) only. Changes in PGC number were measured on different days according to developmental stages.
RESULTS
In male right gonads, but not in female left gonads, at stages 28-30 (incubation 132 h) significant decreases in the mean number of PGCs aggregating were observed compared with the controls (P < 0.05) while the total PGC number in the right and left gonads at each stage did not change (P > 0.05).
CONCLUSION
The present study provides evidence that E(2) has significant effects on the localization of PGCs in male right, but not female left, gonads of chicken embryos at stages 28-30, compared with controls.
目的
评估外源性雌二醇对鸡原始生殖细胞（PGCs）迁移是否有影响。
方法
在X期（孵化第0天）、8 - 10期（孵化30小时）和13 - 15期（孵化55小时），用17β - 雌二醇（E₂）（80微克/枚蛋）处理受精蛋。对照组仅接受溶剂（乳剂）。根据发育阶段在不同天数测量PGC数量的变化。
结果
在28 - 30期（孵化132小时），雄性右侧性腺中聚集的PGC平均数量与对照组相比显著减少（P < 0.05），而雌性左侧性腺未出现此现象，且各阶段左右性腺中PGC总数未发生变化（P > 0.05）。
结论
本研究提供的证据表明，与对照组相比，E₂对28 - 30期鸡胚雄性右侧而非雌性左侧性腺中PGC的定位有显著影响。
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