Prasad Sahdeo, Kalra Neetu, Singh Madhulika, Shukla Yogeshwer
Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, M.G.Marg, Lucknow, India.
Asian J Androl. 2008 Mar;10(2):313-8. doi: 10.1111/j.1745-7262.2008.00313.x. Epub 2007 Dec 20.
To investigate antioxidant potential of lupeol/mango pulp extract (MPE) in testosterone induced oxidative stress in prostate of male Swiss albino mice.
Oral treatment of lupeol (1 mg/animal) and MPE (1 mL [20% w/v]/animal) was given separately to animals along with subcutaneous injection of testosterone (5 mg/kg body weight) consecutively for 15 days. At the end of the study period, the prostate was dissected out for the determination of reactive oxygen species (ROS) levels, lipid peroxidation and antioxidant enzymes status (catalase, superoxide dismutase, glutathione reductase, glutathione-S-transferase).
In testosterone treated animals, increased ROS resulted in depletion of antioxidant enzymes and increase in lipid peroxidation in mouse prostate. However, lupeol/MPE treatment resulted in a decrease in ROS levels with restoration in the levels of lipid peroxidation and antioxidant enzymes.
The results of the present study demonstrate that lupeol/MPE are effective in combating oxidative stress-induced cellular injury of mouse prostate. Mango and its constituents, therefore, deserve study as a potential chemopreventive agent against prostate cancer.
研究羽扇豆醇/芒果果肉提取物（MPE）对雄性瑞士白化小鼠睾丸素诱导的前列腺氧化应激的抗氧化潜力。
分别给动物口服羽扇豆醇（1毫克/只动物）和MPE（1毫升[20%重量/体积]/只动物），并连续15天皮下注射睾丸素（5毫克/千克体重）。在研究期结束时，解剖取出前列腺，用于测定活性氧（ROS）水平、脂质过氧化和抗氧化酶状态（过氧化氢酶、超氧化物歧化酶、谷胱甘肽还原酶、谷胱甘肽-S-转移酶）。
在接受睾丸素治疗的动物中，ROS增加导致小鼠前列腺中抗氧化酶耗竭以及脂质过氧化增加。然而，羽扇豆醇/MPE治疗导致ROS水平降低，脂质过氧化和抗氧化酶水平恢复。
本研究结果表明，羽扇豆醇/MPE可有效对抗氧化应激诱导的小鼠前列腺细胞损伤。因此，芒果及其成分值得作为前列腺癌潜在化学预防剂进行研究。
