Albrecht Amy L, Somji Seema, Sens Mary Ann, Sens Donald A, Garrett Scott H
Department of Pathology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58202, USA.
Biometals. 2008 Aug;21(4):405-16. doi: 10.1007/s10534-007-9129-0. Epub 2007 Dec 21.
The human prostate gland undergoes a prominent alteration in Zn+2 homeostasis during the development of prostate cancer. The goal of the present study was to determine if the immortalized human prostate cell line (RWPE-1) could serve as a model system to study the role of zinc in prostate cancer. The study examined the expression of mRNA for 19 members of the zinc transporter gene family in normal prostate tissue, the prostate RWPE-1 cell line, and the LNCaP, DU-145 and PC-3 prostate cancer cell lines. The study demonstrated that the expression of the 19 zinc transporters was similar between the RWPE-1 cell line and the in situ prostate gland. Of the 19 zinc transporters, only 5 had levels that were different between the RWPE-1 cells and the tissue samples; all five being increased (ZnT-6, Zip-1, Zip-3A, Zip-10, and Zip-14). The response of the 19 transporters was also determined when the cell lines were exposed to 75 microM Zn+2 for 24 h. It was shown for the RWPE-1 cells that only 5 transporters responded to Zn+2 with mRNA for ZnT-1 and ZnT-2 being increased while mRNA for ZnT-7, Zip-7 and Zip-10 transporters were decreased. It was shown for the LNCaP, DU-145 and PC-3 cells that Zn+2 had no effect on the mRNA levels of all 19 transporters except for an induction of ZnT-1 in PC-3 cells. Overall, the study suggests that the RWPE-1 cells could be a valuable model for the study of the zinc transporter gene family in the prostate.
在前列腺癌发展过程中，人类前列腺腺体的锌离子（Zn²⁺）稳态会发生显著改变。本研究的目的是确定永生化人类前列腺细胞系（RWPE - 1）是否可作为研究锌在前列腺癌中作用的模型系统。该研究检测了锌转运蛋白基因家族19个成员的mRNA在正常前列腺组织、前列腺RWPE - 1细胞系以及LNCaP、DU - 145和PC - 3前列腺癌细胞系中的表达情况。研究表明，RWPE - 1细胞系与原位前列腺腺体中19种锌转运蛋白的表达相似。在这19种锌转运蛋白中，只有5种在RWPE - 1细胞和组织样本之间的表达水平存在差异；这5种蛋白的表达均上调（锌转运蛋白6、锌铁转运蛋白1、锌铁转运蛋白3A、锌铁转运蛋白10和锌铁转运蛋白）。当细胞系暴露于75微摩尔的Zn²⁺中24小时后，还检测了这19种转运蛋白的反应。结果显示，对于RWPE - 1细胞，只有5种转运蛋白对Zn²⁺有反应，其中锌转运蛋白1和锌转运蛋白2的mRNA增加，而锌转运蛋白7、锌铁转运蛋白7和锌铁转运蛋白10的mRNA减少。对于LNCaP、DU - 145和PC - 3细胞，结果显示Zn²⁺对所有19种转运蛋白的mRNA水平均无影响，除了PC - 3细胞中锌转运蛋白1的表达被诱导。总体而言，该研究表明RWPE - 1细胞可能是研究前列腺中锌转运蛋白基因家族的一个有价值的模型。
