Karl Julie A, Wiseman Roger W, Campbell Kevin J, Blasky Alex J, Hughes Austin L, Ferguson Betsy, Read Daniel S, O'Connor David H
Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.
Immunogenetics. 2008 Jan;60(1):37-46. doi: 10.1007/s00251-007-0267-x. Epub 2007 Dec 21.
The rhesus macaque (Macaca mulatta) is an excellent model for human disease and vaccine research. Two populations exhibiting distinctive morphological and physiological characteristics, Indian- and Chinese-origin rhesus macaques, are commonly used in research. Genetic analysis has focused on the Indian macaque population, but the accessibility of these animals for research is limited. Due to their greater availability, Chinese rhesus macaques are now being used more frequently, particularly in vaccine and biodefense studies, although relatively little is known about their immunogenetics. In this study, we discovered major histocompatibility complex (MHC) class I cDNAs in 12 Chinese rhesus macaques and detected 41 distinct Mamu-A and Mamu-B sequences. Twenty-seven of these class I cDNAs were novel, while six and eight of these sequences were previously reported in Chinese and Indian rhesus macaques, respectively. We then performed microsatellite analysis on DNA from these 12 animals, as well as an additional 18 animals, and developed sequence specific primer PCR (PCR-SSP) assays for eight cDNAs found in multiple animals. We also examined our cohort for potential admixture of Chinese and Indian origin animals using a recently developed panel of single nucleotide polymorphisms (SNPs). The discovery of 27 novel MHC class I sequences in this analysis underscores the genetic diversity of Chinese rhesus macaques and contributes reagents that will be valuable for studying cellular immunology in this population.
恒河猴（猕猴）是人类疾病和疫苗研究的优秀模型。在研究中常用的有两个表现出独特形态和生理特征的种群，即印度起源和中国起源的恒河猴。遗传分析主要集中在印度猕猴种群上，但这些动物用于研究的可及性有限。由于中国恒河猴更容易获得，它们现在被更频繁地使用，特别是在疫苗和生物防御研究中，尽管对它们的免疫遗传学了解相对较少。在本研究中，我们在12只中国恒河猴中发现了主要组织相容性复合体（MHC）I类cDNA，并检测到41个不同的Mamu - A和Mamu - B序列。这些I类cDNA中有27个是新发现的，而这些序列中分别有6个和8个先前在中国和印度恒河猴中被报道过。然后，我们对这12只动物以及另外18只动物的DNA进行了微卫星分析，并针对在多只动物中发现的8个cDNA开发了序列特异性引物PCR（PCR - SSP）检测方法。我们还使用最近开发的单核苷酸多态性（SNP）面板检查了我们的队列中中国和印度起源动物的潜在混合情况。在该分析中发现27个新的MHC I类序列，突出了中国恒河猴的遗传多样性，并为研究该种群的细胞免疫学提供了有价值的试剂。
