Shi S R, Liu C, Young L, Taylor Cr
Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California, USA.
Biotech Histochem. 2007 Dec;82(6):301-9. doi: 10.1080/10520290701791763.
A novel protocol for antigen retrieval (AR) for immunohistochemistry (IHC) of retinoblastoma protein (pRB) in formalin fixed, paraffin embedded (FFPE) tissue sections was developed using 0.05% citraconic anhydride as the AR solution for heat treatment based on comparison of different methods. This new protocol has advantages including superior morphological preservation, greater reproducibility, and more intense staining after retrieval. Our study demonstrates the importance of comparing various AR protocols to obtain maximal IHC for standardization and for quantitative IHC.
基于不同方法的比较，开发了一种新的抗原修复（AR）方案，用于福尔马林固定、石蜡包埋（FFPE）组织切片中视网膜母细胞瘤蛋白（pRB）的免疫组织化学（IHC），使用0.05%柠康酸酐作为热处理的AR溶液。该新方案具有多种优势，包括形态保存更佳、重现性更高以及修复后染色更强。我们的研究表明，比较各种AR方案对于实现标准化和定量IHC的最大IHC结果非常重要。
