Department of Oral Biology, Jazan University, Jazan, Saudi Arabia.
Department of Oral Biology, Cairo University, Cairo, Egypt.
F1000Res. 2020 Dec 3;9:1393. doi: 10.12688/f1000research.27186.1. eCollection 2020.
This study was conducted to evaluate the role of exogenous epidermal growth factor (EGF) injection on the Ki-67 immuno-expression in submandibular salivary gland tissue of rats receiving doxorubicin (DXR). A total of 21 two-month-old male albino rats, of 200 g body weight, were divided into three groups: control group; DXR group, the rats received 20 mg/kg body weight DXR as a single intra peritoneal injection; DXR+EGF group, the rats received the same dose of DXR and on the next day they were injected intraperitoneally with 10 µg/kg body weight of EGF daily for one week. Histological sections and immunohistochemical expression of Ki67 sections were examined using a ZEISS Primo Star light microscopy and images taken using Tucsen IS 1000 10.0MP Camera. Ki-67 expression was significantly increased in submandibular salivary glands of rats after DXR injection. However, Ki-67 expression in the glandular tissue was restored to normal levels after EGF injection. EGF preserved glandular architecture after DXR injection and maintained Ki-67 immune-expression within the glandular tissue near to the normal level.
本研究旨在评估外源性表皮生长因子（EGF）注射在接受多柔比星（DXR）的大鼠下颌下腺组织中 Ki-67 免疫表达中的作用。共 21 只 2 月龄雄性白化大鼠，体重 200g，分为三组：对照组；DXR 组，大鼠接受 20mg/kg 体重 DXR 单次腹腔注射；DXR+EGF 组，大鼠接受相同剂量的 DXR，第二天腹腔内注射 10µg/kg 体重 EGF，每日一次，共一周。使用 ZEISS Primo Star 光显微镜检查组织学切片和 Ki67 免疫组化表达，并使用 Tucsen IS 1000 10.0MP 相机拍摄图像。DXR 注射后，大鼠下颌下腺的 Ki-67 表达明显增加。然而，EGF 注射后，腺组织中的 Ki-67 表达恢复正常水平。EGF 保存了 DXR 注射后的腺结构，并使 Ki-67 免疫表达保持在接近正常水平的腺组织内。
