Cardoso Juliana F, Souza Bruna R, Amadeu Thaís P, Valença Samuel S, Porto Luís Cristóvão M S, Costa Andréa M A
Histology and Embryology Department, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Toxicol Pathol. 2007 Dec;35(7):890-6. doi: 10.1080/01926230701459986.
It has been clinically and experimentally shown that cigarette smokers suffer from impaired wound healing, but the mechanisms that lead to the alterations are not well understood. The aim of this study was to investigate if the effects of cigarette smoke exposure on excisional cutaneous wound healing are different depending on the strain (Swiss, BALB/c and C57BL/6 mice) studied. Male mice were exposed to smoke of nine whole cigarettes per day, 3 times/day, daily, for 10 days. In the 11th day a full-thickness excisional wound was performed. Control group was sham-exposed and also had a full-thickness excisional wound. The cigarette smoke exposure protocol was performed until euthanasia. Animals were euthanatized 14 days after wounding. Wound contraction was evaluated 7 and 14 days after lesion. Sections were stained with hematoxylin-eosin, Sirius red or toluidine blue and immunostained for alpha-smooth muscle actin. Smoke exposed animals presented delay in wound contraction, in fibroblastic and inflammatory cells recruitment and in myofibroblastic differentiation; those alterations were strain dependent. Cigarette smoke exposure also affected mast cells recruitment and neoepidermis thickness. In conclusion, the present study demonstrated that the effects of cigarette smoke in mice cutaneous wound healing are related to mice strain studied.
临床和实验均已表明，吸烟者的伤口愈合受损，但其导致这种改变的机制尚不清楚。本研究的目的是调查根据所研究的品系（瑞士小鼠、BALB/c小鼠和C57BL/6小鼠）不同，香烟烟雾暴露对切除性皮肤伤口愈合的影响是否存在差异。雄性小鼠每天暴露于9支整支香烟的烟雾中，每天3次，持续10天。在第11天进行全层切除伤口手术。对照组进行假暴露处理，同样进行全层切除伤口手术。香烟烟雾暴露方案持续至安乐死。在受伤14天后对动物实施安乐死。在损伤后7天和14天评估伤口收缩情况。切片用苏木精-伊红、天狼星红或甲苯胺蓝染色，并进行α-平滑肌肌动蛋白免疫染色。暴露于烟雾的动物在伤口收缩、成纤维细胞和炎性细胞募集以及肌成纤维细胞分化方面出现延迟；这些改变具有品系依赖性。香烟烟雾暴露还影响肥大细胞募集和新表皮厚度。总之，本研究表明香烟烟雾对小鼠皮肤伤口愈合的影响与所研究的小鼠品系有关。
