一种可靠的用于检测人血清中利福平、红霉素、林可霉素、氯霉素和阿米卡星的两小时葡萄球菌平板试验。

A reliable two-hour Staphylococcus plate assay of rifampicin, erythromycin, lincomycin, chloramphenicol and amikacin in human sera.

作者信息

Fanaki N H, el-Nakeeb M A

机构信息

Department of Pharmaceutical Microbiology, Alexandria University, Egypt.

出版信息

J Chemother. 1991 Oct;3(5):310-4. doi: 10.1080/1120009x.1991.11739111.

DOI:10.1080/1120009x.1991.11739111

PMID:1809810

Abstract

A simple and precise agar diffusion microassay was developed for rifampicin (RIF) in serum. The method involved the addition of sera containing RIF (0.125-4.0 micrograms/ml) to wells cut in an agar medium (pH 6.6) surface seeded with an adjusted inoculum of Staphylococcus aureus ATCC 6538 P and incubated for 2h at 40 degrees C. Repetitive assays of sera containing known concentrations of RIF revealed an average mean recovery of 93.6% with a coefficient of variation of 5.7%. This rapid method allowed accurate determination of RIF in the presence of erythromycin, chloramphenicol, lincomycin and amikacin. These four antibiotics were also separately measured in sera by the rapid assay at different pH values.

摘要

开发了一种简单精确的琼脂扩散微量测定法，用于测定血清中的利福平（RIF）。该方法包括将含有利福平（0.125 - 4.0微克/毫升）的血清加入到在pH值为6.6的琼脂培养基表面切割的孔中，该琼脂培养基表面接种了经调整的金黄色葡萄球菌ATCC 6538 P接种物，并在40℃下孵育2小时。对含有已知浓度利福平的血清进行重复测定，平均回收率为93.6%，变异系数为5.7%。这种快速方法能够在存在红霉素、氯霉素、林可霉素和阿米卡星的情况下准确测定利福平。还通过在不同pH值下的快速测定法分别测定了血清中的这四种抗生素。