Yao Yanyan, Ohko Yoshihisa, Sekiguchi Yuki, Fujishima Akira, Kubota Yoshinobu
Department of Urology and Molecular Science, Graduate School of Medicine, Yokohama City University, 3-9 Fukuura, Yokohama 236-0004, Japan.
J Biomed Mater Res B Appl Biomater. 2008 May;85(2):453-60. doi: 10.1002/jbm.b.30965.
Ag/titanium dioxide (TiO(2))-coated silicon catheters were easily fabricated with Ag nanoparticles deposition on both the inside wall and the outside wall of TiO(2)-coated catheters by TiO(2) photocatalysis. This is an application of the silicon catheters coated with TiO(2), which possess a self-sterilizing and self-cleaning property combining with UV light illumination (Ohko et al., J Biomed Mater Res: Appl Biomater 2001;58:97). Ag/TiO(2)-coated silicon catheters exhibited a strong bactericidal effect even in the dark. When the 2-5 x 10(5) of colony-forming units of Escherichia coli, Pseudomonas aeruginosa, or Staphylococcus aureus were respectively applied to the surface of the Ag/TiO(2) catheters, which were loaded with approximately 15 nmol cm(-2) of Ag, 99% effective sterilization occurred in a very short time: 20 min for E. coli, 60 min for P. aeruginosa, and 90 min for S. aureus. Additionally, the Ag/TiO(2)-coated catheters possessed a strong self-cleaning property. Using UV illumination, the photocatalytic decomposition rate of methylene blue dye representing the self-cleaning capability, on an Ag/TiO(2) catheter which was loaded with 2 nmol cm(-2) of Ag, was approximately 1.2 times higher (at maximum) than that on TiO(2) coating alone. Furthermore, the Ag nanoparticles can be pre-eminently and uniformly deposited onto the TiO(2) coating, and the amount of Ag was easily controllable from a few nanomoles per square centimeter to approximately 70 nmol cm(-2) by changing the UV illumination time for TiO(2) photocatalysis. This type of catheter shows a great promise in lowering the incidence of catheter-related bacterial infections.
通过二氧化钛光催化作用，将银纳米颗粒沉积在二氧化钛涂层导管的内壁和外壁上，可轻松制备银/二氧化钛（TiO₂）涂层硅导管。这是对涂有TiO₂的硅导管的一种应用，该导管具有与紫外线照射相结合的自消毒和自清洁特性（大光等，《生物医学材料研究杂志：应用生物材料》2001年；58:97）。即使在黑暗中，银/TiO₂涂层硅导管也表现出很强的杀菌效果。当分别将2 - 5×10⁵个菌落形成单位的大肠杆菌、铜绿假单胞菌或金黄色葡萄球菌应用于负载约15 nmol/cm²银的银/TiO₂导管表面时，在很短时间内即可实现99%的有效杀菌：大肠杆菌20分钟，铜绿假单胞菌60分钟，金黄色葡萄球菌90分钟。此外，银/TiO₂涂层导管具有很强的自清洁特性。使用紫外线照射时，负载2 nmol/cm²银的银/TiO₂导管上代表自清洁能力的亚甲基蓝染料的光催化分解速率（最高）比仅涂有TiO₂的导管高出约1.2倍。此外，银纳米颗粒可以卓越且均匀地沉积在TiO₂涂层上，通过改变TiO₂光催化的紫外线照射时间，银的含量可轻松控制在每平方厘米几纳摩尔至约70 nmol/cm²之间。这种类型的导管在降低导管相关细菌感染的发生率方面显示出巨大的前景。
