Department of Microbiology, Faculty of Biological Sciences, University of Wroclaw, 50-137 Wroclaw, Poland.
Department and Clinic of Psychiatry, Wroclaw Medical University, 50-367 Wroclaw, Poland.
Int J Mol Sci. 2021 Dec 28;23(1):284. doi: 10.3390/ijms23010284.
The aim of this study was to assess the beneficial inhibitory effect of silver nanoparticles immobilized on SiO or TiO on biofilm formation by -one of the most dangerous pathogens isolated from urine and bronchoalveolar lavage fluid of patients hospitalized in intensive care units. Pure and silver doped nanoparticles of SiO and TiO were prepared using a novel modified sol-gel method. Ten clinical strains of and the reference PAO1 strain were used. The minimal inhibitory concentration (MIC) was determined by the broth microdilution method. The minimal biofilm inhibitory concentration (MBIC) and biofilm formation were assessed by colorimetric assay. Bacterial enumeration was used to assess the viability of bacteria in the biofilm. Silver nanoparticles immobilized on the SiO and TiO indicated high antibacterial efficacy against planktonic and biofilm cultures. TiO/Ag showed a better bactericidal effect than SiO/Ag. Our results indicate that the inorganic compounds (SiO, TiO) after nanotechnological modification may be successfully used as antibacterial agents against multidrug-resistant strains.
本研究旨在评估固定在 SiO 或 TiO 上的银纳米粒子对生物膜形成的有益抑制作用，供体是从重症监护病房住院患者的尿液和支气管肺泡灌洗液中分离出的一种最危险的病原体之一。使用新型改良溶胶-凝胶法制备了纯 SiO 和 TiO 以及掺银纳米粒子。使用了十种临床分离株和参考 PAO1 菌株。通过肉汤微量稀释法测定最小抑菌浓度（MIC）。通过比色法评估最小生物膜抑制浓度（MBIC）和生物膜形成。细菌计数用于评估生物膜中细菌的活力。固定在 SiO 和 TiO 上的银纳米粒子对浮游和生物膜培养的具有高度的抗菌功效。TiO/Ag 比 SiO/Ag 具有更好的杀菌效果。我们的结果表明，经过纳米技术修饰的无机化合物（SiO、TiO）可成功用作针对多药耐药 菌株的抗菌剂。
