Yedgar S, Gatt S
Biochemistry. 1976 Jun 15;15(12):2570-3. doi: 10.1021/bi00657a013.
Mixed dispersions of the nonionic detergent Triton X-100 and sphingomyelin were used as substrate for sphingomyelinase of rat brain. The dependence of the rate of hydrolysis on the concentration of sphingomyelin was measured in two ways: at a fixed concentration of Triton X-100 or at varying concentrations of this detergent, while maintaining a fixed molar ratio of Triton X-100 to sphingomyelin. In either case, the upsilon vs. S curves deviated from the hyperbolic shape predicted by the Michaelis-Menten kinetic theory. These deviations are discussed and interpreted on the basis of the physicochemical properties of the mixed dispersions of detergent and lipid studied in previous papers.
非离子型去污剂Triton X - 100和鞘磷脂的混合分散液被用作大鼠脑鞘磷脂酶的底物。通过两种方式测量水解速率对鞘磷脂浓度的依赖性：在Triton X - 100浓度固定时，或在该去污剂浓度变化时，同时保持Triton X - 100与鞘磷脂的固定摩尔比。在任何一种情况下，υ对S曲线都偏离了米氏动力学理论预测的双曲线形状。根据先前论文中研究的去污剂和脂质混合分散液的物理化学性质，对这些偏差进行了讨论和解释。
