Kuo W N, Shoji M, Kuo J F
Biochim Biophys Acta. 1976 Jun 23;437(1):142-9. doi: 10.1016/0304-4165(76)90355-x.
The crude protein kinase modulator preparations obtained from several rat tissues (aorta, brain heart, liver, lung, skeletal muscle, small intestine and testis) were separated into their stimulatory and inhibitory modulator components by Sephadex G-100 gel filtration. The isolated stimulatory modulator augmented the activity of guanosine 3':5'-monophosphate-dependent protein kinase. The isolated inhibitory modulator, on the other hand, depressed the activity of cyclic AMP-dependent protein kinase; it was without effect on the activity of cyclic GMP-dependent protein kinease. The present findings indicate that in the mammal, apparently in contrast to the arthropoda, separate proteins are responsibile for the stimulatory and the inhibitory activities of protein kinase modulator and that the two classes of cyclic nucleotide-dependent protein kinase are regulated in an opposing manner by these two types of modulators.
从几只大鼠组织（主动脉、脑、心脏、肝脏、肺、骨骼肌、小肠和睾丸）中获得的粗制蛋白激酶调节剂制剂，通过Sephadex G - 100凝胶过滤被分离成其刺激和抑制调节剂成分。分离出的刺激调节剂增强了3':5'-环磷酸鸟苷依赖性蛋白激酶的活性。另一方面，分离出的抑制调节剂降低了环磷酸腺苷依赖性蛋白激酶的活性；它对环磷酸鸟苷依赖性蛋白激酶的活性没有影响。目前的研究结果表明，在哺乳动物中，显然与节肢动物不同，蛋白激酶调节剂的刺激和抑制活性由不同的蛋白质负责，并且这两类环核苷酸依赖性蛋白激酶受到这两种类型调节剂的相反调节。
