自旋标记高铁血红蛋白的电子自旋共振光谱的温度依赖性
Temperature dependence of ESR spectra of spin-labelled met-hemoglobin.
作者信息
Nöthig-Laslo V, Herak J N, Maricić S
出版信息
Biophys Chem. 1976 May;4(3):281-5. doi: 10.1016/0301-4622(76)80076-2.
PMID:181098
Abstract
The maleimide spin-label, firmly fixed to the protein, was used to study conformation changes of various met-hemoglobin derivatives. The temperature dependence of the rotational correlation time shows a distinct conformation change in aquomet-hemoglobin at about 25 degrees C. The other met-hemoglobin derivatives studied (fluoro-, cyano-, aquomet-complexed with inositol hexaphosphate) and carbonmonoxy-hemoglobin exhibit no conformation changes in the temperature range from 0-50 degrees C.
摘要
固定在蛋白质上的马来酰亚胺自旋标记物用于研究各种高铁血红蛋白衍生物的构象变化。旋转相关时间的温度依赖性表明，在约25℃时，高铁血红蛋白水溶液的构象发生明显变化。所研究的其他高铁血红蛋白衍生物（氟代、氰代、与肌醇六磷酸复合的高铁血红蛋白水溶液）以及一氧化碳血红蛋白在0至50℃的温度范围内未表现出构象变化。
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