Saha N, Hong S H, Wong H A, Jeyaseelan K, Tay J S
Department of Paediatrics, National University of Singapore.
Jinrui Idengaku Zasshi. 1991 Dec;36(4):307-12. doi: 10.1007/BF01883603.
Biochemical characteristics of one non-deficient fast G6PD variant (GdSingapore) and six different deficient variants (three new, two Mahidol, one each of Indonesian and Mediterranean) were studied among the Malays of Singapore. The GdSingapore variant had normal enzyme activity (82%) and fast electrophoretic mobilities (140% in TEB buffer, 160% in phosphate and 140% in Tris-HCl buffer systems respectively). This variant is further characterized by normal Km for G6P; utilization of analogues (Gal6P, 2dG6P; dAmNADP), heat stability and pH optimum. The other six deficient G6PD variants had normal electrophoretic mobility in TEB buffer with enzyme activities ranging from 1 to 12% of GdB+. The biochemical characteristics identity them to be 2 Mahidol, 1 Indonesian and 1 Mediterranean variants and three new deficient variants.
在新加坡马来人群体中，研究了一种非缺陷型快速G6PD变体（Gd新加坡型）和六种不同的缺陷型变体（三种新变体、两种马希多尔型、一种印度尼西亚型和一种地中海型）的生化特性。Gd新加坡型变体具有正常的酶活性（82%）和快速的电泳迁移率（在TEB缓冲液中为140%，在磷酸盐缓冲液中为160%，在Tris-HCl缓冲液系统中分别为140%）。该变体的进一步特征包括对G6P的正常Km值；对类似物（Gal6P、2dG6P；dAmNADP）的利用、热稳定性和最适pH值。其他六种缺陷型G6PD变体在TEB缓冲液中具有正常的电泳迁移率，酶活性范围为GdB+的1%至12%。生化特性表明它们分别为2种马希多尔型、1种印度尼西亚型和1种地中海型变体以及三种新的缺陷型变体。
