Sunaga H, Yamane Y, Aoki Y, Suzuki K T
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Res Commun Chem Pathol Pharmacol. 1991 Nov;74(2):215-22.
Distribution of cadmium (Cd) in liver supernatant was examined by gel filtration chromatography after injecting Cd intravenously into female Wistar rats and also by adding Cd in vitro into the control supernatant. Supernatants were analyzed on an HPLC Asahipak GST-520 column and on-line ICP (inductively coupled argon plasma-atomic emission spectrometer) analysis of the eluate (HPLC-ICP). The relative intensity of the three main Cd-associated peaks changed with time after injection of Cd into rats. The three peaks were tentatively assigned as Cd-binding protein-I and -II (Cd-BP-I and -II) and metallothionein (MT) in the order of respective elution from the column. Relative affinities of Cd for the three peaks were determined by injecting different doses of Cd into rats as follows: MT greater than Cd-BP-II greater than Cd-BP-I. Cd-BP-II was assigned as the major Cd-BP other than MT in the liver supernatant and it contained zinc that can be replaced by Cd. Comparison between the in vivo and in vitro Cd distributions indicated that the former distribution is not reproducible under any in vitro conditions examined and the latter distribution is dependent mostly upon incubation temperature.
通过向雌性Wistar大鼠静脉注射镉（Cd），以及在体外向对照上清液中添加镉，利用凝胶过滤色谱法检测肝脏上清液中镉的分布情况。上清液在HPLC Asahipak GST - 520柱上进行分析，并对洗脱液进行在线电感耦合氩等离子体原子发射光谱仪（ICP）分析（HPLC - ICP）。向大鼠注射镉后，三个主要镉相关峰的相对强度随时间变化。这三个峰按照从柱中洗脱的顺序暂定为镉结合蛋白 - I和 - II（Cd - BP - I和 - II）以及金属硫蛋白（MT）。通过向大鼠注射不同剂量的镉来确定镉对这三个峰的相对亲和力如下：MT大于Cd - BP - II大于Cd - BP - I。Cd - BP - II被确定为肝脏上清液中除MT之外的主要镉结合蛋白，并且它含有可被镉取代的锌。体内和体外镉分布的比较表明，前者的分布在任何检测的体外条件下都不可重现，而后者的分布主要取决于孵育温度。
